[鸡脂肪酸结合蛋白基因的克隆与测序]

[Cloning and sequencing of fatty acid binding proteins gene in chicken].

作者信息

Wang Qi-Gui, Li Ning, Deng Xue-Mei, Gu Zhi-Liang, Liu Zhao-Liang, Li Hui, Chen Zhi-Qiang, Lian Zheng-Xing, Wu Chang-Xin

机构信息

National Laboratories for Agribiotechnology, China Agricultural University, Beijing 100094, China.

出版信息

Yi Chuan Xue Bao. 2002 Feb;29(2):115-8.

PMID:11901992

Abstract

A pair of primers was designed according to the sequence of mammal fatty acid binding protein (FABP) gene, then PCR amplified to chicken genome. After the product of PCR was cloned and sequenced, homologous comparison was done among porcine heart fatty acid binding protein gene and porcine adipocyte fatty acid binding protein gene. The result showed that the sequence of chicken FABP gene had 68% and 75% homology with porcine H-FABP and A-FABP gene respectively, and had 75% homology with porcine AFABP on amino acid level. The result of Northern showed that the gene only expressed in fat tissues.

摘要

根据哺乳动物脂肪酸结合蛋白（FABP）基因序列设计一对引物，然后对鸡基因组进行PCR扩增。PCR产物克隆测序后，对猪心脏脂肪酸结合蛋白基因和猪脂肪细胞脂肪酸结合蛋白基因进行同源性比较。结果表明，鸡FABP基因序列与猪H-FABP和A-FABP基因的同源性分别为68%和75%，在氨基酸水平上与猪AFABP的同源性为75%。Northern杂交结果表明该基因仅在脂肪组织中表达。