Zargar Seyed Jalal, Rabbani Azra
Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, Iran.
Int J Biol Macromol. 2002 Apr 8;30(2):113-7. doi: 10.1016/s0141-8130(02)00009-0.
Using ultraviolet spectroscopy and equilibrium dialysis techniques, we have investigated the interaction of anticancer drug, daunomycin with calf thymus histone H(1) chromosomal protein in 20 mM phosphate buffer, pH 7.0, 1 mM EDTA at room temperature. The UV spectroscopy results show that daunomycin (5.0-100 microM) decreases the absorbance of histone H(1) at 210-230 nm and induces hypochromicity in the absorption spectrum of the protein. The equilibrium dialysis data show that daunomycin binds to histone H(1) and the binding process is positive cooperative with two binding sites as Scatchard plot and Hill coefficient confirm it. The results suggest that daunomycin binds to histone H(1) and changes its conformation.
利用紫外光谱和平衡透析技术，我们研究了抗癌药物柔红霉素与小牛胸腺组蛋白H(1)染色体蛋白在室温下于pH 7.0的20 mM磷酸盐缓冲液、1 mM EDTA中的相互作用。紫外光谱结果表明，柔红霉素（5.0 - 100 microM）降低了组蛋白H(1)在210 - 230 nm处的吸光度，并在蛋白质的吸收光谱中诱导了减色效应。平衡透析数据表明，柔红霉素与组蛋白H(1)结合，且结合过程具有正协同性，有两个结合位点，斯卡查德图和希尔系数证实了这一点。结果表明，柔红霉素与组蛋白H(1)结合并改变其构象。
