Fedotov N S, Panchenko L F, Tarshis M A
Folia Microbiol (Praha). 1975;20(6):488-95. doi: 10.1007/BF02891708.
Membrane vesicles obtained from Acholeplasma laidlawii accumulate glucose as well as maltose and fructose against their concentration gradient in the absence of exogenous energy sources. Glucose uptake by membrane vesicles is inhibited by anaerobiosis and by electron transfer inhibitors, such as rotenone and amytal, but not by 2-heptyl-4-hydroxyquinoline N-oxide, antimycin A, cyanide and azide. Rotenone, cyanide and amytal also produce a rapid efflux of glucose from the membrane vesicles. Arsenate, oligomycin and N,N'-dicyclohexylcarbodimide do not inhibit glucose transport. Transport of glucose is markedly inhibited by proton conductors such as CCCP and pentachlorophenol. It is concluded that glucose transport can be driven by a high-energy state of the membrane or by the membrane potential.
从莱氏无胆甾原体获得的膜泡在没有外源能源的情况下，能逆浓度梯度积累葡萄糖以及麦芽糖和果糖。膜泡对葡萄糖的摄取受厌氧状态和电子传递抑制剂（如鱼藤酮和阿米妥）的抑制，但不受2-庚基-4-羟基喹啉N-氧化物、抗霉素A、氰化物和叠氮化物的抑制。鱼藤酮、氰化物和阿米妥也会使葡萄糖从膜泡中快速外流。砷酸盐、寡霉素和N,N'-二环己基碳二亚胺不抑制葡萄糖转运。质子导体如羰基氰氯苯腙和五氯苯酚能显著抑制葡萄糖转运。得出的结论是，葡萄糖转运可以由膜的高能状态或膜电位驱动。
