Petersen B, Gernaey K, Vanrolleghem P A
BIOMATH, Ghent University, Gent, Belgium.
Water Sci Technol. 2002;45(4-5):181-90.
An experimental procedure for anoxic activated sludge monitoring with combined nitrate and titrimetric measurements is proposed and evaluated successfully with two known carbon sources, acetate and dextrose. For nitrate measurements an ion-selective nitrate electrode is applied to allow for frequent measurements, and thereby the possibility for detailed determination of the denitrification biokinetics. An internal nitrate electrode calibration is implemented in the experiments to avoid the often-encountered electrode drift problem. It was observed that the best experimental design was with the carbon source in excess, since excess nitrate provoked nitrite build-up thereby complicating the data interpretation. A conceptual model could quantitatively describe the experimental observations and thus link the experimentally measured proton production with the consumption of electron acceptor and carbon source during denitrification.
提出了一种结合硝酸盐和滴定法测量来监测缺氧活性污泥的实验方法，并使用两种已知碳源（乙酸盐和葡萄糖）成功进行了评估。对于硝酸盐测量，应用离子选择性硝酸盐电极以进行频繁测量，从而有可能详细确定反硝化生物动力学。实验中进行了内部硝酸盐电极校准，以避免经常遇到的电极漂移问题。观察到最佳实验设计是碳源过量，因为过量硝酸盐会导致亚硝酸盐积累，从而使数据解释复杂化。一个概念模型可以定量描述实验观察结果，从而将实验测量的质子产生与反硝化过程中电子受体和碳源的消耗联系起来。
