Páli Tibor, Marsh Derek
Institute of Biophysics, Biological Research Centre, P.O.Box 521, 6701 Szeged, Hungary.
Cell Mol Biol Lett. 2002;7(1):87-91.
Non-linear electron spin resonance (EPR) techniques suitable for measuring proximity relationships in membranes are reviewed. These were developed during the past decade in order to measure changes sensitively in the spin-lattice relaxation time (T1) of nitroxyl spin labels covalently attached to membrane lipids or proteins. In combination with paramagnetic quenching agents and double spin-labelling, the methods were further developed for distance measurements. Selected examples are given to illustrate different methods, and types of data obtained for both integral and peripheral membrane proteins.
本文综述了适用于测量膜中邻近关系的非线性电子自旋共振（EPR）技术。这些技术是在过去十年中开发的，用于灵敏地测量共价连接到膜脂或蛋白质上的硝酰自旋标记的自旋 - 晶格弛豫时间（T1）的变化。结合顺磁猝灭剂和双自旋标记，这些方法进一步发展用于距离测量。给出了选定的示例来说明不同的方法，以及针对整合膜蛋白和外周膜蛋白获得的数据类型。
