[钙调神经磷酸酶在血管紧张素II刺激的心脏成纤维细胞增殖中的作用]

[Role of calcineurin in cardiac fibroblast proliferation stimulated by angiotensin II].

作者信息

Xu S, Fu M G, Xu Y F, Pang Y Z, Tang C S

机构信息

Institute of Cardiovascular Research, the First Hospital, Beijing Medical University, Beijing 100034, China.

出版信息

Sheng Li Xue Bao. 2000 Aug;52(4):305-7.

PMID:11951112

Abstract

The present study was undertaken to observe the role of calcineurin (CaN) in the angiotensin II (Ang II) stimulated cardiac fibroblast proliferation. In cultured cardiac fibroblasts of neonatal rats, Ang II was used to stimulate proliferation while CaN was inhibited by CaN CsA inhibitor cyclosporin A (CsA). (3)H-TdR incorporation, activity of CaN and intracellular calcium concentration were measured. (3)H-TdR incorporation of Ang II stimulated fibroblasts was 72% higher than control (P<0.01), which was inhibited by CsA (0.1 10 micromol/L) in a dose dependent manner. Intracellular Ca(2+) level and CaN activity of Ang II stimulated fibroblasts were respectively 112% (P<0.01) and 17%(P<0.05) higher than control. It is concluded that CaN may play an important role in signal transduction of the Ang II induced cardiac fibroblast proliferation.

摘要

本研究旨在观察钙调神经磷酸酶（CaN）在血管紧张素II（Ang II）刺激的心脏成纤维细胞增殖中的作用。在新生大鼠培养的心脏成纤维细胞中，用Ang II刺激增殖，同时用CaN抑制剂环孢素A（CsA）抑制CaN。测量了（3）H-TdR掺入量、CaN活性和细胞内钙浓度。Ang II刺激的成纤维细胞的（3）H-TdR掺入量比对照组高72%（P<0.01），并被CsA（0.1~10 μmol/L）以剂量依赖方式抑制。Ang II刺激的成纤维细胞的细胞内Ca（2+）水平和CaN活性分别比对照组高112%（P<0.01）和17%（P<0.05）。结论是，CaN可能在Ang II诱导的心脏成纤维细胞增殖的信号转导中起重要作用。