Begum Aynun Nahar, Terao Junji
Department of Nutrition, School of Medicine, The University of Tokushima, Japan.
Biosci Biotechnol Biochem. 2002 Feb;66(2):398-403. doi: 10.1271/bbb.66.398.
Alpha-tocotrienol (alpha-T3) has been suggested to protect cellular membranes against free radical damage. This study was done to estimate the effect of alpha-T3 on free radical-induced impairment of erythrocyte deformability by comparing it to alpha-tocopherol (alpha-T). An erythrocyte suspension containing 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) was forced to flow through microchannels with an equivalent diameter of 7 microm for measuring erythrocyte deformability. A higher concentration of AAPH caused a marked decrease in erythrocyte deformability with concomitant increase of membranous lipid peroxidation. Treatment of erythrocytes with alpha-T or alpha-T3 suppressed the impairment of erythrocyte deformability as well as membranous lipid peroxidation and they also increased erythrocyte deformability even in the absence of AAPH. In these cases, the protecting effect of alpha-T3 was significantly higher than that of alpha-T. We emphasize that higher incorporating activity of alpha-T3 into erythrocyte membranes seems to be the most important reason for higher protection against erythrocyte oxidation and impairment its deformability.
已有人提出α-生育三烯酚（α-T3）可保护细胞膜免受自由基损伤。本研究旨在通过将α-T3与α-生育酚（α-T）进行比较，评估其对自由基诱导的红细胞变形性损伤的影响。含有2,2'-偶氮二（2-脒基丙烷）二盐酸盐（AAPH）的红细胞悬液被迫流经等效直径为7微米的微通道，以测量红细胞变形性。较高浓度的AAPH会导致红细胞变形性显著降低，同时膜脂质过氧化增加。用α-T或α-T3处理红细胞可抑制红细胞变形性损伤以及膜脂质过氧化，并且即使在没有AAPH的情况下，它们也能增加红细胞变形性。在这些情况下，α-T3的保护作用明显高于α-T。我们强调，α-T3更高的掺入红细胞膜的活性似乎是其对红细胞氧化和变形性损伤具有更高保护作用的最重要原因。
