Pronuclear abnormalities and cytoskeletal organization during assisted fertilization in a patient with multifollicular ovarian response.
作者信息
Rawe V Y, Kopelman S, Nodar F N, Olmedo S Brugo, Chillik C F
机构信息
Centro de Estudios en Ginecología y Reproducción, CEGyR, Viamonte, Buenos Aires, Argentina.
出版信息
J Assist Reprod Genet. 2002 Mar;19(3):152-7. doi: 10.1023/a:1014745006944.
PURPOSE
To analyze the distribution of a tubulins and acetylated alpha tubulins and the chromatin configuration in abnormally fertilized zygotes from a patient with a multifollicular ovarian response after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).
METHODS
Immunofluorescence and phase contrast microscopy was performed in abnormally fertilized zygotes.
RESULTS
After phase contrast microscopy analysis, immunofluorescence staining was performed in 20 oocytes that developed > or = 3 pronuclei (PN) and karyomeres after IVF-ICSI. Around 80% of the abnormal zygotes from IVF were the consequence of monospermic fertilizations. Retention of the second polar body (PB) and the presumptive split of > or =1 PN within the cytoplasm were the main events present in most oocytes after IVF-ICSI.
CONCLUSIONS
Fluorescence labeling of selected sperm and oocyte components affords a unique view of abnormal fertilized zygotes. Surprisingly, anomalies detected after IVF-ICSI showed similar etiologies in this special group of zygotes.
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