具有不同亲和力的受体介导海马神经元中的时相性和紧张性GABA(A)电导。
Receptors with different affinities mediate phasic and tonic GABA(A) conductances in hippocampal neurons.
作者信息
Stell Brandon M, Mody Istvan
机构信息
Department of Neurology, University of California Los Angeles School of Medicine, Los Angeles, California 90095, USA.
出版信息
J Neurosci. 2002 May 15;22(10):RC223. doi: 10.1523/JNEUROSCI.22-10-j0003.2002. Epub 2002 May 10.
A tonic inhibitory conductance mediated by GABA(A) receptors that supplements the phasic inhibition produced by IPSCs has been identified in the hippocampus and cerebellum. It is presently unknown whether tonic and phasic inhibitions are mediated by GABA(A) receptors with different subunit assemblies. Here we demonstrate that a low concentration (200 nm) of the highly specific competitive GABA(A) antagonist SR95531 (gabazine) reduces phasic inhibition in hippocampal granule cells by 71% but has no effect on tonic inhibition, whereas a high concentration (10 microm) of the antagonist blocked both conductances. These findings are consistent with tonic and phasic conductances being mediated by different GABA(A) receptor subtypes with different affinities for GABA.
在海马体和小脑中已发现一种由GABA(A)受体介导的强直抑制性电导，它补充了由抑制性突触后电流产生的相位抑制。目前尚不清楚强直抑制和相位抑制是否由具有不同亚基组合的GABA(A)受体介导。在这里，我们证明，低浓度（200 nM）的高特异性竞争性GABA(A)拮抗剂SR95531（gabazine）可使海马颗粒细胞的相位抑制降低71%，但对强直抑制无影响，而高浓度（10 μM）的该拮抗剂则可阻断这两种电导。这些发现与强直电导和相位电导由对GABA具有不同亲和力的不同GABA(A)受体亚型介导一致。
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