Squires D J, Lamerton L F
Br J Haematol. 1975 Jan;29(1):31-42. doi: 10.1111/j.1365-2141.1975.tb01797.x.
Two diffusion chamber assays, termed the 'Full" chamber technique and the 'Empty' chamber technique, have been used to determine the effect of various doses of cyclophosphamide, vinblastine and busulphan on the population of diffusion chamber progenitor cells (DCPC). The diffusion chamber dose-response curves were compared to the progenitor cell survivals estimated by the spleen colony technique. The in vitro agar colony assay was also performed on the busulphan-treated marrow. The diffusion chamber and spleen colony techniques estimated similar survivals after cyclophosphamide and vinblastine treatment. However, with busulphan, the chamber, spleen colony and agar colony methods estimated different survivals. The indication is that the diffusion chamber techniques assay the same population of cells as the spleen colony technique, and that busulphan alters the capacity of surviving DCPC to generate granulocytes and macrophages.
两种扩散室试验，即“全”室技术和“空”室技术，已被用于确定不同剂量的环磷酰胺、长春碱和白消安对扩散室祖细胞（DCPC）群体的影响。将扩散室剂量反应曲线与通过脾集落技术估计的祖细胞存活率进行比较。还对白消安处理的骨髓进行了体外琼脂集落试验。环磷酰胺和长春碱处理后，扩散室和脾集落技术估计的存活率相似。然而，对于白消安，扩散室、脾集落和琼脂集落方法估计的存活率不同。这表明扩散室技术与脾集落技术检测的是同一细胞群体，并且白消安改变了存活的DCPC产生粒细胞和巨噬细胞的能力。
