利用蚀刻和镀银玻璃纤维尖端对生物材料进行拉曼光谱研究。

Raman spectroscopy investigation of biological materials by use of etched and silver coated glass fiber tips.

作者信息

Gessner R, Rösch P, Kiefer W, Popp J

机构信息

Institute for Physical Chemistry, Am Hubland, Würzburg D-97074, Germany.

出版信息

Biopolymers. 2002;67(4-5):327-30. doi: 10.1002/bip.10090.

DOI:10.1002/bip.10090

PMID:12012459

Abstract

The results for surface enhanced Raman scattering (SERS) studies on biological samples are reported. Etched and silver coated glass fiber tips were used as a SERS substrate. This method enabled the recording of spectra of biological samples, such as plant tissue or microbiological cells, with a high spatial resolution. Because of the low laser power used with the fiber tips, it was even possible to investigate tissues that are very sensitive toward laser power as it is used in a common micro-Raman setup.

摘要

报告了对生物样品进行表面增强拉曼散射（SERS）研究的结果。蚀刻并镀银的玻璃纤维尖端用作SERS基底。该方法能够以高空间分辨率记录生物样品（如植物组织或微生物细胞）的光谱。由于纤维尖端使用的激光功率较低，甚至有可能研究在普通显微拉曼装置中对激光功率非常敏感的组织。