唾液酸对纤维状胶原蛋白基因表达的影响：正常和纤维化瘢痕来源的成纤维细胞中的不同变化。
The effects of sialic acid on the gene expression of fibrillar collagens: different changes in normal and fibrotic scar derived fibroblasts.
作者信息
Kössi J, Aalto J, Haataja S, Niinikoski J, Peltonen J, Laato M
机构信息
Department of Surgery, University of Turku, Kiinamyllynkatu 4-8, 20520 Turku, Finland.
出版信息
Ann Chir Gynaecol Suppl. 2001(215):25-8.
OBJECTIVE
To investigate the effect of free sialic acid on collagen gene expression in fibroblasts.
DESIGN
Cell culture study.
SETTING
University hospital, Finland. CELL LINES: Human granulation tissue fibroblasts, human hypertrophic scar fibroblasts and human keloid fibroblasts.
INTERVENTIONS
Treatment of cell cultures with 3 microM, 30 microM and 300 microM N-acetyl-neuraminic acid.
MAIN OUTCOME MEASURES
The measurement of steady state level of mRNA for type I and type III collagen.
RESULTS
Fibroblast lines react dissimilarly under the influence of sialic acid. Granulation tissue fibroblasts showed decrease in the gene expression of type I and III collagen, while keloid fibroblasts contrastingly showed an increase. Hypertrophic scar derived fibroblasts showed no change.
CONCLUSIONS
Sialic acids may decrease collagen gene expression in granulation tissue and that disturbed wound healing in diabetics and smokers may in part be due to direct effect of sialic acids on fibroblasts. Sialic acids may in part induce keloid formation.
目的
研究游离唾液酸对成纤维细胞中胶原蛋白基因表达的影响。
设计
细胞培养研究。
单位
芬兰大学医院。
细胞系
人肉芽组织成纤维细胞、人增生性瘢痕成纤维细胞和人瘢痕疙瘩成纤维细胞。
干预措施
用3微摩尔、30微摩尔和300微摩尔的N - 乙酰神经氨酸处理细胞培养物。
主要观察指标
I型和III型胶原蛋白mRNA稳态水平的测定。
结果
在唾液酸的影响下，成纤维细胞系反应不同。肉芽组织成纤维细胞I型和III型胶原蛋白的基因表达降低，而瘢痕疙瘩成纤维细胞则相反，表达增加。增生性瘢痕来源的成纤维细胞无变化。
结论
唾液酸可能降低肉芽组织中的胶原蛋白基因表达，糖尿病患者和吸烟者伤口愈合受干扰可能部分归因于唾液酸对成纤维细胞的直接作用。唾液酸可能部分诱导瘢痕疙瘩形成。
Zotero 插件