Kössi J, Muona P, Tuukkanen J, Ylä-Outinen H, Kalliomäki M, Risteli J, Oikarinen A, Laato M, Peltonen J
Department of Surgery, University of Turku, Turku, Finland.
Ann Chir Gynaecol Suppl. 2001(215):39-44.
Diabetes mellitus (DM) is a complex metabolic disease associated with increased accumulation of extracellular matrix by endothelial cells and contributing to vascular complications of long-standing diabetes. On the other hand, DM is also associated with decreased accumulation of extracellular matrix in granulation tissue, which is suggested to be a consequence of impaired angiogenesis. The role of hyperglycemia in these situations is not fully understood. We examined the effects of high glucose concentrations on the gene expression and secretion of various collagens in cultured EAhy 926 endothelial cells. EAhy 926 endothelial cells expressed alpha 1(I) collagen mRNA at a low level and small amount of the corresponding peptide was secreted from the cells; mRNA was not affected but peptide secretion was increased by elevated glucose concentration. mRNAs for type III and VI collagens were not detected in the endothelial cells Furthermore, high glucose concentration in long term had no morphological effects on cultured endothelial cells but increased the expression of type IV collagen, which could rather be beneficial for angiogenesis in a healing wound. Our results suggest that high glucose concentration per se may contribute to increased accumulation of extracellular matrix in blood vessels but probably is not responsible for decreased angiogenesis and granulation tissue formation in diabetic patients.
糖尿病（DM）是一种复杂的代谢性疾病，与内皮细胞胞外基质积累增加相关，并导致长期糖尿病的血管并发症。另一方面，DM还与肉芽组织中胞外基质积累减少有关，这被认为是血管生成受损的结果。高血糖在这些情况下的作用尚未完全了解。我们研究了高糖浓度对培养的EAhy 926内皮细胞中各种胶原蛋白基因表达和分泌的影响。EAhy 926内皮细胞低水平表达α1（I）型胶原蛋白mRNA，细胞分泌少量相应肽；mRNA不受影响，但葡萄糖浓度升高会增加肽的分泌。在内皮细胞中未检测到III型和VI型胶原蛋白的mRNA。此外，长期高糖浓度对培养的内皮细胞没有形态学影响，但增加了IV型胶原蛋白的表达，这可能对愈合伤口中的血管生成有益。我们的结果表明，高糖浓度本身可能导致血管中胞外基质积累增加，但可能与糖尿病患者血管生成减少和肉芽组织形成无关。
