[气相色谱 - 电子捕获检测器法测定人血浆中 felodipine 浓度及其药代动力学研究]

[Determination of felodipine concentration in human plasma by GC-ECD and study of its pharmacokinetics].

作者信息

Yang L, Yuan Y, Dai X

机构信息

Department of Instrumental Analysis, Nanjing General Hospital of PLA, Nanjing 210002.

出版信息

Yao Xue Xue Bao. 1998 Jun;33(6):461-4.

PMID:12016918

Abstract

A rapid, accurate and sensitive gas chromatographic method using electron-capture detector (GC-ECD) was developed for the determination of felodipine concentration in human plasma. Nimodipine was used as internal standard. The plasma samples were extracted using a mixture of ethyl ether and n-hexane (2:1) to give mean recoveries of 97.3% of felodipine. The extracts were separated by a 5% SE-30 glass packed column (2 m x 3 mm). The detection limits for felodipine was 0.25 ng.ml-1. The precisions (RSD%, n = 5) of within-day and day-to-day were less than 5.09% and 8.62%, respectively. The pharmacokinetic parameters of felodipine in ten men were investigated by this method. The concentration-time curve was fitted to a two-compartment model. Its main pharmacokinetic parameters were: T1/2 alpha = 0.76 h, T1/2 beta = 16.09 h, Cmax = 4.78 ng.ml-1, Tmax = 2.01 h, AUC = 45.2 h.ng.ml-1.

摘要

建立了一种使用电子捕获检测器（GC-ECD）的快速、准确且灵敏的气相色谱法，用于测定人血浆中费洛地平的浓度。尼莫地平用作内标。血浆样品用乙醚和正己烷（2:1）的混合物萃取，费洛地平的平均回收率为97.3%。萃取物通过5% SE-30玻璃填充柱（2 m×3 mm）进行分离。费洛地平的检测限为0.25 ng·ml-1。日内和日间精密度（RSD%，n = 5）分别小于5.09%和8.62%。用该方法研究了10名男性受试者体内费洛地平的药代动力学参数。浓度-时间曲线拟合为二室模型。其主要药代动力学参数为：T1/2α = 0.76 h，T1/2β = 16.09 h，Cmax = 4.78 ng·ml-1，Tmax = 2.01 h，AUC = 45.2 h·ng·ml-1。