He Biao, Adler Paul N
Biology Department and Cancer Center, Gilmer Hall, University of Virginia, Charlottesville, VA 22903, USA.
BMC Dev Biol. 2002 May 3;2:7. doi: 10.1186/1471-213x-2-7.
The frizzled pathway in Drosophila has been studied intensively for its role in the development of planar polarity in wing hairs, thoracic bristles and ommatidia. Selected cells in the arista (the terminal segment of the antenna) elaborate a lateral projection that shares characteristics with both hairs and bristles.
We found that mutations in putative downstream members of the frizzled pathway, such as inturned resulted in multipled and split laterals, but no obvious polarity defects. Mutations in upstream genes such as frizzled did not show an adult arista phenotype. When we examined lateral development in the mutants we found that, as is the case on the wing for hair development the frizzled pathway regulated the subcellular location for lateral initiation. However, on the arista an altered location for lateral initiation did not result in altered polarity, as did an altered site for hair initiation. The regulation of lateral development involved the preferential accumulation of Frizzled protein at the distal edges of lateral cells much as is seen on the wing. In contrast to the situation in wing cells, in arista cells the location for lateral initiation was close to but did not overlap the region of preferential Frizzled accumulation.
Our data indicates that a modified version of the frizzled pathway regulates arista development. We conclude that the lack of a polarity defect in mutant aristae is likely to be a consequence of inherent differences in the cell biology of wing hair and lateral forming cells.
果蝇中的卷曲蛋白信号通路已被深入研究，因其在翅毛、胸毛和小眼平面极性发育中的作用。触角末端节（触角芒）中的特定细胞形成一种侧向突起，其兼具毛和刚毛的特征。
我们发现，卷曲蛋白信号通路假定下游成员的突变，如inturned突变，会导致多个和分裂的侧向突起，但无明显的极性缺陷。卷曲蛋白等上游基因的突变未表现出成虫触角芒表型。当我们检查突变体中的侧向发育时，发现正如翅上毛的发育情况一样，卷曲蛋白信号通路调节侧向起始的亚细胞定位。然而，在触角芒上，侧向起始位置的改变并未像毛起始位置改变那样导致极性改变。侧向发育的调节涉及卷曲蛋白在侧向细胞远端边缘的优先积累，这与翅上的情况类似。与翅细胞不同的是，在触角芒细胞中，侧向起始位置靠近但不与卷曲蛋白优先积累区域重叠。
我们的数据表明，卷曲蛋白信号通路的一种修饰形式调节触角芒发育。我们得出结论，突变触角芒缺乏极性缺陷可能是翅毛和侧向形成细胞的细胞生物学固有差异的结果。
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