Mizumoto Kazuhiro, Tanaka Masao
Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
J Hepatobiliary Pancreat Surg. 2002;9(1):39-44. doi: 10.1007/s005340200003.
Genetic analysis of pancreatic juice is a promising aid for the accurate and early diagnosis of pancreatic cancer. K- ras mutation is frequently observed in pancreatic cancer; however, it is not specific for carcinoma because pancreatic adenoma and pancreatitis also show this mutation. Overexpression of p53 protein is solely detected in pancreatic juice from pancreatic cancer patients, but the positivity rate differs among various reports. Telomerase activity in pancreatic juice was detected in 20 of 24 (83.3%) pancreatic cancer patients and in only 1 of 23 (4.3%) pancreatic adenoma patients, while none of 23 (0%) pancreatitis patients showed evident telomerase activity. The relative value for telomerase activity was significantly higher in pancreatic cancer than in adenoma and pancreatitis. Centrosome abnormalities are very frequently seen in pancreas cancer tissues and the detection of these abnormalities is expected to be a potent new diagnostic tool for the genetic analysis of pancreatic juice. Genetic analysis of pancreatic juice will improve the sensitivity and specificity of pancreatic cancer diagnosis.
胰液的基因分析对于胰腺癌的准确早期诊断是一种很有前景的辅助手段。K-ras突变在胰腺癌中经常被观察到；然而，它并非癌特异性的，因为胰腺腺瘤和胰腺炎也会出现这种突变。仅在胰腺癌患者的胰液中检测到p53蛋白的过表达，但不同报告中的阳性率有所不同。在24例胰腺癌患者中的20例（83.3%）检测到胰液中的端粒酶活性，而23例胰腺腺瘤患者中只有1例（4.3%）检测到，23例胰腺炎患者中无一例（0%）表现出明显的端粒酶活性。胰腺癌中端粒酶活性的相对值显著高于腺瘤和胰腺炎。中心体异常在胰腺癌组织中非常常见，检测这些异常有望成为胰液基因分析的一种有效的新诊断工具。胰液的基因分析将提高胰腺癌诊断的敏感性和特异性。
