基质细胞衍生因子1α蛋白在HIV脑炎中的表达

Expression of stromal cell-derived factor 1alpha protein in HIV encephalitis.

作者信息

Langford Dianne, Sanders Virginia J, Mallory Margaret, Kaul Markus, Masliah Eliezer

机构信息

Department of Pathology, School of Medicine, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0624, USA.

出版信息

J Neuroimmunol. 2002 Jun;127(1-2):115-26. doi: 10.1016/s0165-5728(02)00068-1.

DOI:10.1016/s0165-5728(02)00068-1

PMID:12044982

Abstract

Analysis of the patterns of stromal cell-derived factor 1alpha (SDF-1alpha) expression in the brains from HIV-positive patients suggests that in neuronal cells, SDF-1alpha might play a role in neuroprotection and neurite extension in response to HIV infection. In all cases analyzed, SDF-1alpha immunoreactivity was primarily present in astroglial cells. Patients with HIV encephalitis (HIVE) showed intense somato-dendritic neuronal SDF-1alpha immunoreactivity, while HIVE negative patients with neurodegeneration had a significant decrease in neuronal SDF-1alpha immunoreactivity. Neuronal cells treated with SDF-1alpha displayed increased neurite outgrowth. Similarly, neurons treated with HIV-Tat, which induced SDF-1alpha expression, also showed neurite outgrowth. Tat-mediated neurite outgrowth was blocked by anti-SDF-1alpha antibody. These results suggest that SDF-1alpha may play a role in the neuronal response to HIV in the brains of AIDS patients.

摘要

对HIV阳性患者大脑中基质细胞衍生因子1α（SDF-1α）表达模式的分析表明，在神经元细胞中，SDF-1α可能在响应HIV感染时发挥神经保护和神经突延伸的作用。在所有分析的病例中，SDF-1α免疫反应主要存在于星形胶质细胞中。患有HIV脑炎（HIVE）的患者显示出强烈的体树突状神经元SDF-1α免疫反应，而患有神经退行性变的HIVE阴性患者的神经元SDF-1α免疫反应显著降低。用SDF-1α处理的神经元细胞显示出神经突生长增加。同样，用诱导SDF-1α表达的HIV-Tat处理的神经元也显示出神经突生长。Tat介导的神经突生长被抗SDF-1α抗体阻断。这些结果表明，SDF-1α可能在艾滋病患者大脑中神经元对HIV的反应中发挥作用。