Flt-3配体（FL）可驱动表达OX62和/或CD161（自然杀伤细胞受体P1）的大鼠骨髓来源树突状细胞的分化。

Flt-3 ligand (FL) drives differentiation of rat bone marrow-derived dendritic cells expressing OX62 and/or CD161 (NKR-P1).

作者信息

Brissette-Storkus Cynthia S, Kettel J C, Whitham T F, Giezeman-Smits K M, Villa L A, Potter D M, Chambers William H

机构信息

Eye and Ear Institute and Department of Ophthalmology, Brain Tumor Center of the University of Pittsburgh Cancer Institute, Pennsylvania 15213, USA.

出版信息

J Leukoc Biol. 2002 Jun;71(6):941-9.

PMID:12050178

Abstract

Bone marrow-derived dendritic cells (DC) of the rat have not been as well characterized as those from the mouse. Here, large quantities of bone marrow-derived rat DC were generated when Flt-3 ligand (FL) was used as an adjunct to granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). These cells displayed a typical DC phenotype, expressing MHC class II, CD54, CD80, CD86, and CD11b/c. These DC also uniformly expressed low levels of CD161 and expressed OX62 in a bimodal distribution. Few cells were recovered from cultures grown without FL, and they failed to express OX62 or CD161. The DC generated with FL were more potent antigen-presenting cells in mixed lymphocyte cultures than cells grown without FL, and among FL-derived cells, the OX62+ cells were slightly more stimulatory than OX62- cells. Thus, FL is a useful cytokine for obtaining large quantities of functional rat DC subsets in vitro.

摘要

大鼠骨髓来源的树突状细胞（DC）尚未像小鼠的那样得到充分表征。在此，当将Flt-3配体（FL）用作粒细胞巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-4（IL-4）的辅助剂时，可产生大量大鼠骨髓来源的DC。这些细胞表现出典型的DC表型，表达MHC II类、CD54、CD80、CD86和CD11b/c。这些DC还均一性地低水平表达CD161，并以双峰分布表达OX62。在无FL的培养物中回收的细胞很少，且它们不表达OX62或CD161。与无FL培养的细胞相比，用FL产生的DC在混合淋巴细胞培养物中是更强效的抗原呈递细胞，并且在FL来源的细胞中，OX62+细胞比OX62-细胞的刺激作用稍强。因此，FL是一种用于在体外获得大量功能性大鼠DC亚群的有用细胞因子。