Marra C A, Zaidenberg A, de Alaniz M J T, Buschiazzo H
INIBIOLP (Instituto de Investigaciones Bioquímicas de La Plata), Cátedra de Bioquímica, Universidad Nacional de La Plata, Calle 60 y 120, 1900, Argentina.
Ann Trop Med Parasitol. 2002 Apr;96(3):249-64. doi: 10.1179/000349802125000781.
The fatty-acid composition of liver lipids from mice infected with Trypanosoma cruzi (clone H510C8C3) or uninfected mice was investigated. The infected animals were treated orally for 30 days, with trifluralin (TFL) or benznidazole (BNZ), each at 100mg/kg.day, or only with the peanut oil used as the drug vehicle. The uninfected mice were also given the peanut oil. The treatments were stopped 10 days before the animals were killed. The liver microsomal lipids of each mouse were isolated and then analysed by gas-liquid chromatography. In terms of the total lipids, untreated infection evoked a significant increase in saturated fatty acids and the members of the n-9 fatty-acid family, with a concomitant decrease in the polyenoates of the n-3 and n-6 fatty-acid series. Each lipid subclass was affected to a different extent, the phospholipids being affected most. All lipid fractions, apart from the cholesterol esters, showed a significant increase in the proportion of n-9 isomers. Infection also produced a marked increase in the absolute amounts of triacylglycerides, cholesterol and cholesterol esters in liver microsomal membranes. After BNZ or TFL treatment, the fatty-acid pattern of mice that had been infected was indistinguishable from that of the control mice. The possible role of desaturase activity in the alterations observed is discussed.
对感染克氏锥虫（克隆H510C8C3）的小鼠或未感染小鼠肝脏脂质的脂肪酸组成进行了研究。对感染动物口服三氟拉林（TFL）或苄硝唑（BNZ），剂量均为100mg/kg·天，持续30天，或仅给予用作药物载体的花生油。未感染小鼠也给予花生油。在处死动物前10天停止治疗。分离每只小鼠的肝脏微粒体脂质，然后通过气液色谱法进行分析。就总脂质而言，未经治疗的感染导致饱和脂肪酸和n-9脂肪酸家族成员显著增加，同时n-3和n-6脂肪酸系列的多烯酸减少。每个脂质亚类受到的影响程度不同，磷脂受到的影响最大。除胆固醇酯外，所有脂质组分中n-9异构体的比例均显著增加。感染还导致肝脏微粒体膜中甘油三酯、胆固醇和胆固醇酯的绝对量显著增加。经BNZ或TFL治疗后，感染小鼠的脂肪酸模式与对照小鼠无法区分。讨论了去饱和酶活性在观察到的变化中的可能作用。
