肿瘤坏死因子α刺激人肠道Caco-2细胞中的牛磺酸摄取及转运体基因表达。

Tumor necrosis factor alpha stimulates taurine uptake and transporter gene expression in human intestinal Caco-2 cells.

作者信息

Mochizuki Tetsunosuke, Satsu Hideo, Shimizu Makoto

机构信息

Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, Japan.

出版信息

FEBS Lett. 2002 Apr 24;517(1-3):92-6. doi: 10.1016/s0014-5793(02)02584-x.

DOI:10.1016/s0014-5793(02)02584-x

PMID:12062416

Abstract

The effect of cytokines on the taurine uptake by human intestinal epithelial Caco-2 cells was investigated. Among the various cytokines tested, tumor necrosis factor alpha (TNF-alpha) markedly increased the taurine uptake by Caco-2 cells, resulting in an increase in the intracellular taurine level. TNF-alpha did not induce up-regulation of the taurine uptake in hepatic HepG2, renal human embryo kidney 293, and macrophage-like THP-1 cells. The uptake of glycine, L-leucine, and L-glutamic acid by Caco-2 cells was not affected by TNF-alpha. A kinetic analysis of the taurine uptake by TNF-alpha-treated Caco-2 cells suggests that this up-regulation was associated with both an increase in the amount of the taurine transporter (TAUT) and an increase in its affinity. TNF-alpha-treated cells showed a higher mRNA level of the TAUT than did the control cells.

摘要

研究了细胞因子对人肠上皮Caco-2细胞摄取牛磺酸的影响。在测试的各种细胞因子中，肿瘤坏死因子α（TNF-α）显著增加了Caco-2细胞对牛磺酸的摄取，导致细胞内牛磺酸水平升高。TNF-α并未诱导肝HepG2细胞、肾人胚肾293细胞和巨噬细胞样THP-1细胞中牛磺酸摄取的上调。TNF-α对Caco-2细胞摄取甘氨酸、L-亮氨酸和L-谷氨酸没有影响。对TNF-α处理的Caco-2细胞摄取牛磺酸的动力学分析表明，这种上调与牛磺酸转运体（TAUT）数量的增加及其亲和力的提高有关。TNF-α处理的细胞比对照细胞显示出更高的TAUT mRNA水平。