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从姜黄中提取的姜黄素的辐射防护作用:对γ射线照射诱导的尿8-羟基-2'-脱氧鸟苷形成、肿瘤发生的抑制作用,但对死亡率无影响。

Radioprotective action of curcumin extracted from Curcuma longa LINN: inhibitory effect on formation of urinary 8-hydroxy-2'-deoxyguanosine, tumorigenesis, but not mortality, induced by gamma-ray irradiation.

作者信息

Inano Hiroshi, Onoda Makoto

机构信息

Redox Regulation Research Group, Research Center for Radiation Safety, National Institute of Radiological Sciences, 9-1 Anagawa-4-chome, Inage-ku, Chiba-shi 263-8555, Japan.

出版信息

Int J Radiat Oncol Biol Phys. 2002 Jul 1;53(3):735-43. doi: 10.1016/s0360-3016(02)02794-3.

Abstract

PURPOSE

We evaluated the radioprotective action of curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione] extracted from Curcuma longa LINN against the acute and chronic effects and the mortality induced by exposure to radiation using female rats.

METHODS AND MATERIALS

For the assay of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in urine, a marker for acute effects, Wistar-MS virgin rats were fed the basal diet with exposure at 0 or 3 Gy to gamma-rays from a 60Co source as the control. Rats in the experimental groups received whole-body irradiation with 3 Gy and were fed a diet containing 1% (wt/wt) curcumin for 3 days before and/or 2 days after irradiation. The urine was collected for a 24-h period between 1 and 2 days after irradiation. Urine samples were used to determine the 8-OHdG level using an enzyme-linked immunosorbent assay and the creatinine level by a modified Jaffé reaction. For long-term effects, rats at Day 17 of pregnancy were fed a diet containing curcumin for 3 days before and/or 3 days after irradiation with 1.5 Gy, and received a pellet of diethylstilbestrol as the promoter. The rats were examined for mammary and pituitary tumors for 1 year. To determine survival, virgin rats received whole-body irradiation with 9.6 Gy and were fed a diet containing curcumin for 3 days before and/or 3 days after irradiation. After irradiation, all rats were assessed daily for survival for 30 days.

RESULTS

Acutely in virgin rats irradiated with 3 Gy, the creatinine-corrected concentration and total amount of 8-OHdG in the 24-h urine samples were higher (approximately 1.3-fold) than the corresponding values in the nonirradiated controls. Adding curcumin to the diet for 3 days before and/or 2 days after irradiation reduced the elevated 8-OHdG levels by 50-70%. The evaluation of the protective action of curcumin against the long-term effects revealed that curcumin significantly decreased the incidence of mammary and pituitary tumors. However, the experiments on survival revealed that curcumin was not effective when administered for 3 days before and/or 3 days after irradiation (9.6 Gy).

CONCLUSION

These findings demonstrate that curcumin can be used as an effective radioprotective agent to inhibit acute and chronic effects, but not mortality, after irradiation.

摘要

目的

我们评估了从姜黄中提取的姜黄素[1,7-双(4-羟基-3-甲氧基苯基)-1,6-庚二烯-3,5-二酮]对雌性大鼠辐射所致急性和慢性影响以及死亡率的辐射防护作用。

方法和材料

为检测作为急性效应标志物的尿中8-羟基-2'-脱氧鸟苷(8-OHdG),将Wistar-MS初产大鼠喂食基础饮食,分别接受0或3 Gy来自60Co源的γ射线照射作为对照。实验组大鼠接受3 Gy全身照射,并在照射前3天和/或照射后2天喂食含1%(重量/重量)姜黄素的饮食。在照射后1至2天期间收集24小时尿液。尿液样本用于通过酶联免疫吸附测定法测定8-OHdG水平,并通过改良的贾菲反应测定肌酐水平。对于长期影响,怀孕第17天的大鼠在接受1.5 Gy照射前3天和/或照射后3天喂食含姜黄素的饮食,并接受己烯雌酚丸剂作为启动剂。对大鼠进行1年的乳腺和垂体肿瘤检查。为确定存活率,初产大鼠接受9.6 Gy全身照射,并在照射前3天和/或照射后3天喂食含姜黄素的饮食。照射后,每天评估所有大鼠30天的存活率。

结果

在接受3 Gy照射的初产大鼠中,24小时尿液样本中经肌酐校正的8-OHdG浓度和总量比未照射对照中的相应值高(约1.3倍)。在照射前3天和/或照射后2天的饮食中添加姜黄素可使升高的8-OHdG水平降低50 - 70%。对姜黄素长期影响防护作用的评估显示,姜黄素显著降低了乳腺和垂体肿瘤的发生率。然而,存活率实验显示,在照射(9.6 Gy)前3天和/或照射后3天给予姜黄素无效。

结论

这些发现表明,姜黄素可作为一种有效的辐射防护剂,用于抑制照射后的急性和慢性影响,但不能降低死亡率。

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