Inano Hiroshi, Onoda Makoto
Redox Regulation Research Group, Research Center for Radiation Safety, National Institute of Radiological Sciences, 9-1 Anagawa-4-chome, Inage-ku, Chiba-shi 263-8555, Japan.
Int J Radiat Oncol Biol Phys. 2002 Jul 1;53(3):735-43. doi: 10.1016/s0360-3016(02)02794-3.
We evaluated the radioprotective action of curcumin [1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione] extracted from Curcuma longa LINN against the acute and chronic effects and the mortality induced by exposure to radiation using female rats.
For the assay of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in urine, a marker for acute effects, Wistar-MS virgin rats were fed the basal diet with exposure at 0 or 3 Gy to gamma-rays from a 60Co source as the control. Rats in the experimental groups received whole-body irradiation with 3 Gy and were fed a diet containing 1% (wt/wt) curcumin for 3 days before and/or 2 days after irradiation. The urine was collected for a 24-h period between 1 and 2 days after irradiation. Urine samples were used to determine the 8-OHdG level using an enzyme-linked immunosorbent assay and the creatinine level by a modified Jaffé reaction. For long-term effects, rats at Day 17 of pregnancy were fed a diet containing curcumin for 3 days before and/or 3 days after irradiation with 1.5 Gy, and received a pellet of diethylstilbestrol as the promoter. The rats were examined for mammary and pituitary tumors for 1 year. To determine survival, virgin rats received whole-body irradiation with 9.6 Gy and were fed a diet containing curcumin for 3 days before and/or 3 days after irradiation. After irradiation, all rats were assessed daily for survival for 30 days.
Acutely in virgin rats irradiated with 3 Gy, the creatinine-corrected concentration and total amount of 8-OHdG in the 24-h urine samples were higher (approximately 1.3-fold) than the corresponding values in the nonirradiated controls. Adding curcumin to the diet for 3 days before and/or 2 days after irradiation reduced the elevated 8-OHdG levels by 50-70%. The evaluation of the protective action of curcumin against the long-term effects revealed that curcumin significantly decreased the incidence of mammary and pituitary tumors. However, the experiments on survival revealed that curcumin was not effective when administered for 3 days before and/or 3 days after irradiation (9.6 Gy).
These findings demonstrate that curcumin can be used as an effective radioprotective agent to inhibit acute and chronic effects, but not mortality, after irradiation.
我们评估了从姜黄中提取的姜黄素[1,7-双(4-羟基-3-甲氧基苯基)-1,6-庚二烯-3,5-二酮]对雌性大鼠辐射所致急性和慢性影响以及死亡率的辐射防护作用。
为检测作为急性效应标志物的尿中8-羟基-2'-脱氧鸟苷(8-OHdG),将Wistar-MS初产大鼠喂食基础饮食,分别接受0或3 Gy来自60Co源的γ射线照射作为对照。实验组大鼠接受3 Gy全身照射,并在照射前3天和/或照射后2天喂食含1%(重量/重量)姜黄素的饮食。在照射后1至2天期间收集24小时尿液。尿液样本用于通过酶联免疫吸附测定法测定8-OHdG水平,并通过改良的贾菲反应测定肌酐水平。对于长期影响,怀孕第17天的大鼠在接受1.5 Gy照射前3天和/或照射后3天喂食含姜黄素的饮食,并接受己烯雌酚丸剂作为启动剂。对大鼠进行1年的乳腺和垂体肿瘤检查。为确定存活率,初产大鼠接受9.6 Gy全身照射,并在照射前3天和/或照射后3天喂食含姜黄素的饮食。照射后,每天评估所有大鼠30天的存活率。
在接受3 Gy照射的初产大鼠中,24小时尿液样本中经肌酐校正的8-OHdG浓度和总量比未照射对照中的相应值高(约1.3倍)。在照射前3天和/或照射后2天的饮食中添加姜黄素可使升高的8-OHdG水平降低50 - 70%。对姜黄素长期影响防护作用的评估显示,姜黄素显著降低了乳腺和垂体肿瘤的发生率。然而,存活率实验显示,在照射(9.6 Gy)前3天和/或照射后3天给予姜黄素无效。
这些发现表明,姜黄素可作为一种有效的辐射防护剂,用于抑制照射后的急性和慢性影响,但不能降低死亡率。