人类NIMA相关蛋白激酶3基因（NEK3）的分子克隆与特性分析

Molecular cloning and characterization of the human NIMA-related protein kinase 3 gene (NEK3).

作者信息

Kimura M, Okano Y

机构信息

Department of Molecular Pathobiochemistry, Gifu University School of Medicine, Gifu, Japan.

出版信息

Cytogenet Cell Genet. 2001;95(3-4):177-82. doi: 10.1159/000059342.

DOI:10.1159/000059342

PMID:12063396

Abstract

NEKs (NIMA-related kinases) are a group of protein kinases sharing high amino acid sequence identities with NIMA (never in mitosis gene a) which control mitosis in Aspergillus nidulans. We have cloned a cDNA for human NEK3, a novel human gene structurally related to NIMA, by RT-PCR. Its open reading frame encodes a protein of 489 amino acid residues with the calculated molecular mass of 56.0 kDa and a predicted pI of 6.58. Phylogenetic analysis suggests that mouse and human NEK3s constitute a subfamily within the NIMA family of protein kinases. The expression pattern of NEK3 was studied by RT-PCR and a high level of expression was detected in testis, ovary, and brain, with low-level expression being detected in most of the tissues studied. NEK3 mRNA was detected in all the proliferating cell lines studied, and the amount did not change during the cell cycle. The human NEK3 gene was assigned to human chromosome 13 by somatic cell hybrids and 13q14.2 by radiation hybrid mapping.

摘要

NEK（NIMA相关激酶）是一组与构巢曲霉中控制有丝分裂的NIMA（有丝分裂中从不表达的基因a）具有高度氨基酸序列同一性的蛋白激酶。我们通过逆转录聚合酶链反应（RT-PCR）克隆了人NEK3的互补DNA（cDNA），NEK3是一个在结构上与NIMA相关的新型人类基因。其开放阅读框编码一个由489个氨基酸残基组成的蛋白质，计算分子量为56.0 kDa，预测的等电点为6.58。系统发育分析表明，小鼠和人类的NEK3在蛋白激酶的NIMA家族中构成一个亚家族。通过RT-PCR研究了NEK3的表达模式，在睾丸、卵巢和大脑中检测到高水平表达，而在大多数所研究的组织中检测到低水平表达。在所研究的所有增殖细胞系中均检测到NEK3信使核糖核酸（mRNA），并且其数量在细胞周期中其含量没有变化。通过体细胞杂种将人类NEK3基因定位于人类13号染色体，通过辐射杂种图谱定位到13q14.2。