Sibileva M A, Zatiaeva A A, Sabaneeva N V, Matveeva N I
St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034 Russia.
Biofizika. 2002 May-Jun;47(3):427-32.
The methods of viscosimetry and flow birefringence were used to study the effect of anions F-, Cl-, Br-, J-, NO3-, ClO4-, SCN-, CH3COO- on the dimensions and thermodynamic rigidity of the DNA molecule in solutions in a wide range of ionic strengths and at different temperatures. It was shown that the persistent lengtH of DNA is independent of the anion type at all values of ionic strength, and the changes in its dimensions, which are determined from the changes in intrinsic viscosity, are due to the influence of anions and their hydration on long-range interactions in the macromolecule. Possible reasons for this phenomena and the role of structural changes in water upon hydration of anions are discussed.
采用粘度测定法和流动双折射法，研究了阴离子F-、Cl-、Br-、I-、NO3-、ClO4-、SCN-、CH3COO-在广泛的离子强度范围和不同温度下对溶液中DNA分子尺寸和热力学刚性的影响。结果表明，在所有离子强度值下，DNA的持久长度均与阴离子类型无关，而由特性粘度变化所确定的其尺寸变化，是由于阴离子及其水合作用对大分子中长程相互作用的影响所致。讨论了这种现象的可能原因以及阴离子水合时水结构变化的作用。
