犬杯状病毒与细胞的早期相互作用是其体外细胞嗜性的主要决定因素。

Early interaction of canine calicivirus with cells is the major determinant for its cell tropism in vitro.

作者信息

Maeda Yasuko, Tohya Yukinobu, Matsuura Yuichi, Mochizuki Masami, Sugimura Takaaki

机构信息

Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University, Japan.

出版信息

Vet Microbiol. 2002 Jul 22;87(4):291-300. doi: 10.1016/s0378-1135(02)00083-4.

DOI:10.1016/s0378-1135(02)00083-4

PMID:12069767

Abstract

Canine calicivirus (CaCV) No. 48 strain isolated from a dog with fatal diarrhea is known to be able to replicate in MDCK and primary dog kidney cells. In this study, two new canine cell lines, MCM-B2 and MCA-B1, were determined to be permissive for CaCV No. 48, whereas other cell lines, including one canine cell line, A-72, were non-permissive. Flow cytometric analysis indicated that CaCV No. 48 binds efficiently to the permissive cells and to some degree also to Vero cells that are non-permissive for the virus, but does not bind to the other non-permissive cells tested. Both the permissive and non-permissive cells could be transfected with genomic RNA from CaCV No. 48, resulting in the appearance of CPE, production of capsid antigen and release of infectious progeny. These results suggested that the early interaction of the virus with cells, probably by binding to a virus receptor on the cell membrane, is the major determinant of CaCV No. 48 cell tropism in vitro.

摘要

从一只患有致命腹泻的狗身上分离出的犬杯状病毒（CaCV）48号毒株，已知能够在犬肾传代细胞（MDCK）和原代犬肾细胞中复制。在本研究中，确定了两种新的犬细胞系MCM - B2和MCA - B1对CaCV 48号毒株敏感，而其他细胞系，包括一种犬细胞系A - 72，则不敏感。流式细胞术分析表明，CaCV 48号毒株能有效地与敏感细胞结合，在一定程度上也能与对该病毒不敏感的非洲绿猴肾细胞（Vero细胞）结合，但不与所检测的其他不敏感细胞结合。敏感细胞和不敏感细胞都可以用CaCV 48号毒株的基因组RNA进行转染，从而导致细胞病变效应的出现、衣壳抗原的产生以及感染性子代病毒的释放。这些结果表明，病毒与细胞的早期相互作用，可能是通过与细胞膜上的病毒受体结合，是CaCV 48号毒株体外细胞嗜性的主要决定因素。