一氧化氮合酶、可卡因和苯丙胺调节转录蛋白以及垂体腺苷酸环化酶激活多肽免疫反应性纤维对豚鼠星状神经节的神经支配。

Innervation of guinea-pig stellate ganglia by nitric oxide synthase, cocaine- and amphetamine-regulated transcript protein- and pituitary adenylate cyclase activating polypeptide-immunoreactive fibers.

作者信息

Locknar S A, Calupca M A, Parsons R L

机构信息

Department of Anatomy and Neurobiology, University of Vermont College of Medicine, Burlington, VT 05405-0068, USA.

出版信息

Neuroscience. 2002;112(3):525-33. doi: 10.1016/s0306-4522(02)00116-1.

DOI:10.1016/s0306-4522(02)00116-1

PMID:12074895

Abstract

The present study analyzed using immunohistochemical labeling the distribution and co-localization of nitric oxide synthase (NOS), cocaine- and amphetamine-regulated transcript peptide (CARTp) and pituitary adenylate cyclase activating polypeptide (PACAP) with choline acetyltransferase (ChAT)-immunoreactive fibers in the guinea-pig stellate ganglia. ChAT-immunoreactive fibers make pericellular baskets around virtually all stellate ganglia neurons. Pericellular baskets of NOS, CARTp and PACAP fibers were also present around numerous stellate ganglia neurons. Although all the NOS and PACAP fibers also exhibited ChAT immunoreactivity, only some of the CARTp fibers were ChAT-immunoreactive. No evidence of co-localization of NOS, PACAP and CARTp was obtained.These results indicate that NOS, PACAP and CARTp are present in distinct preganglionic axons innervating the guinea-pig stellate ganglia.

摘要

本研究采用免疫组织化学标记法，分析了豚鼠星状神经节中一氧化氮合酶（NOS）、可卡因和苯丙胺调节转录肽（CARTp）、垂体腺苷酸环化酶激活多肽（PACAP）与胆碱乙酰转移酶（ChAT）免疫反应性纤维的分布及共定位情况。ChAT免疫反应性纤维几乎围绕着所有星状神经节神经元形成细胞周篮。在众多星状神经节神经元周围也存在NOS、CARTp和PACAP纤维的细胞周篮。虽然所有的NOS和PACAP纤维也显示出ChAT免疫反应性，但只有部分CARTp纤维是ChAT免疫反应性的。未获得NOS、PACAP和CARTp共定位的证据。这些结果表明，NOS、PACAP和CARTp存在于支配豚鼠星状神经节的不同节前轴突中。