Malashenko Iu R, Romanovskaia V A, Bogachenko V N, Shved A D
Mikrobiologiia. 1975 Sep-Oct;44(5):855-62.
Microorganisms assimilating methane at temperatures above 40 degrees C were isolated from various natural sources: ooze, mud, waste water of coal pits. The bacteria are obligate methylotrophs and are represented by two groups: (a) thermotolerant, growing at 37 to 45 degrees C; and (b) thermophilic, growing at 50 to 62 degrees C. The selective factor used to isolate various physiological forms of methylotrophs is corresponding temperatures of growth which allow to isolate from the same substrate meso-, thermotolerant, and thermophilic forms. Morphological and physiological properties of the strains are described. The thermotolerant cultures of methylotrophs are similar to Methylobacter vinelandii, though differ from it by some characteristics. The thermophilic microorganisms should be classed as a separate species Methylococcus thermophilus.
从各种天然来源（软泥、泥浆、煤矿废水）中分离出了在40摄氏度以上温度同化甲烷的微生物。这些细菌是专性甲基营养菌，分为两组：（a）耐热型，在37至45摄氏度下生长；（b）嗜热型，在50至62摄氏度下生长。用于分离甲基营养菌各种生理形态的选择因素是相应的生长温度，这使得能够从同一底物中分离出中温、耐热和嗜热形态。描述了这些菌株的形态和生理特性。甲基营养菌的耐热培养物与维内兰德甲基杆菌相似，但在某些特征上与之不同。嗜热微生物应归类为一个单独的物种——嗜热甲基球菌。
