通过门静脉内注射HVJ阳离子脂质体靶向库普弗细胞。

Kupffer cell targeting by intraportal injection of the HVJ cationic liposome.

作者信息

Yoshida Masanori, Yamamoto N, Uehara T, Terao R, Nitta T, Harada N, Hatano E, Iimuro Y, Yamaoka Y

机构信息

Department of Gastroenterological Surgery, Kyoto University, Graduate School of Medicine, 54 Kawaracho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.

出版信息

Eur Surg Res. 2002 May-Jun;34(3):251-9. doi: 10.1159/000063397.

DOI:10.1159/000063397

PMID:12077513

Abstract

The aim of this study was to target Kupffer cells (KCs) selectively and efficiently by the intraportal injection of fusigenic cationic liposomes with hemagglutinating virus of Japan components (HVJ cationic liposomes). Phosphorothioate FITC-oligodeoxynucleotides (FITC-ODNs) encapsulated in either HVJ cationic liposomes, HVJ anionic liposomes or conventional cationic liposomes without HVJ were transferred to the rat. FITC-ODNs in HVJ cationic liposomes administered via portal vein were selectively transfected to KCs for up to 24 h with no apparent cytotoxicity at higher transfection efficiency than FITC-ODNs in conventional cationic liposomes without HVJ administered via portal vein or tail vein. On the other hand, FITC-ODNs in HVJ anionic liposomes were observed mainly in hepatocytes, not KCs. This new method will be useful for the modulation of KCs activity in both basic research and clinical applications.

摘要

本研究的目的是通过门静脉注射含有日本血凝病毒成分的融合性阳离子脂质体（HVJ阳离子脂质体），选择性且高效地靶向库普弗细胞（KCs）。将包裹在HVJ阳离子脂质体、HVJ阴离子脂质体或不含HVJ的常规阳离子脂质体中的硫代磷酸酯FITC - 寡脱氧核苷酸（FITC - ODNs）转移至大鼠体内。经门静脉给药的HVJ阳离子脂质体中的FITC - ODNs可选择性转染至KCs长达24小时，且在转染效率高于经门静脉或尾静脉给药的不含HVJ的常规阳离子脂质体中的FITC - ODNs时，无明显细胞毒性。另一方面，HVJ阴离子脂质体中的FITC - ODNs主要在肝细胞中观察到，而非KCs。这种新方法在基础研究和临床应用中对调节KCs活性均将是有用的。