Tian W, Wang D
Department of Oral and Maxillofacial Surgery, West China University of Medical Sciences.
Hua Xi Kou Qiang Yi Xue Za Zhi. 1998 Feb;16(1):65-8, 72.
Osteoblast-like cells were isolated from embryonic human calvarium with modified enzyme digestion method. TGF-beta which is believed having biological effects on bone cells was added into the culture medium with separate concentrations. The incorporation of 3H-TdR, 3H-Proline of osteoblast-like cells and alkaline phosphatase(ALP) content of the cells were tested to study the effects of the TGF-beta on the proliferation of osteoblasts. The results showed that TGF-beta increased ALP activity and the synthesis of collagen, and the cell proliferation was inhibited. It affected the proliferation and differentiation of osteoblast-like cells with the best 1 ng/ml among four concentration of 0.01-10 ng/ml. These findings suggested that function of TGF-beta might be modified by other local growth factors.
采用改良酶消化法从人胚胎颅骨中分离出成骨样细胞。将被认为对骨细胞具有生物学作用的转化生长因子-β（TGF-β）以不同浓度添加到培养基中。检测成骨样细胞的3H-胸腺嘧啶核苷（3H-TdR）、3H-脯氨酸掺入量以及细胞碱性磷酸酶（ALP）含量，以研究TGF-β对成骨细胞增殖的影响。结果显示，TGF-β可增加ALP活性和胶原蛋白合成，同时抑制细胞增殖。在0.01 - 10 ng/ml的四个浓度中，1 ng/ml时对成骨样细胞增殖和分化的影响最佳。这些发现提示，TGF-β的功能可能会受到其他局部生长因子的调节。
