Shchelkunov S N, Totmenin A V, Safronov P F, Mikheev M V, Gutorov V V, Ryazankina O I, Petrov N A, Babkin I V, Uvarova E A, Sandakhchiev L S, Sisler J R, Esposito J J, Damon I K, Jahrling P B, Moss B
State Research Center of Virology and Biotechnology Vector, Koltsovo, Novosibirsk Region, Russia.
Virology. 2002 Jun 5;297(2):172-94. doi: 10.1006/viro.2002.1446.
Monkeypox virus (MPV) belongs to the orthopoxvirus genus of the family Poxviridae, is endemic in parts of Africa, and causes a human disease that resembles smallpox. The 196,858-bp MPV genome was analyzed with regard to structural features and open reading frames. Each end of the genome contains an identical but oppositely oriented 6379-bp terminal inverted repetition, which similar to that of other orthopoxviruses, includes a putative telomere resolution sequence and short tandem repeats. Computer-assisted analysis was used to identify 190 open reading frames containing >/=60 amino acid residues. Of these, four were present within the inverted terminal repetition. MPV contained the known essential orthopoxvirus genes but only a subset of the putative immunomodulatory and host range genes. Sequence comparisons confirmed the assignment of MPV as a distinct species of orthopoxvirus that is not a direct ancestor or a direct descendent of variola virus, the causative agent of smallpox.
猴痘病毒(MPV)属于痘病毒科正痘病毒属,在非洲部分地区呈地方性流行,可引起类似天花的人类疾病。对196,858碱基对的猴痘病毒基因组进行了结构特征和开放阅读框分析。基因组的每一端都包含一个相同但方向相反的6379碱基对末端反向重复序列,与其他正痘病毒的类似,其中包括一个假定的端粒分辨率序列和短串联重复序列。采用计算机辅助分析来鉴定190个包含≥60个氨基酸残基的开放阅读框。其中,四个位于反向末端重复序列内。猴痘病毒包含已知的正痘病毒必需基因,但仅包含假定的免疫调节和宿主范围基因的一个子集。序列比较证实猴痘病毒被归为正痘病毒的一个独特物种,它不是天花病原体天花病毒的直接祖先或直接后代。