Waldenström A P, Hjalmarson A C
Recent Adv Stud Cardiac Struct Metab. 1975;10:307-15.
Isolated working rat hearts were made ischemic for 5, 10, and 30 minutes respectively. After the ischemic period, all hearts were perfused in a retrograde nonworking way for 30 minutes. During the 5 first minutes of ischemia, there was a marked fall of cardiac output and coronary flow. A significant release of GOT was seen and this was more marked after longer periods of ischemia. Addition of adrenaline to the perfusate increased the enzyme release. Pacing at 400/minute, high preload, high afterload, acidosis, or alkalosis did not alter enzyme release. Glycogen, ATP and CrP levels were depressed at the end of the ischemic period, but were seen to be rising again during the retrograde perfusion. This study indicates that myocardial tissue may release enzymes without being irreversibly damaged.
将离体工作的大鼠心脏分别进行5分钟、10分钟和30分钟的缺血处理。缺血期过后，所有心脏均以逆行非工作方式灌注30分钟。在缺血的前5分钟内，心输出量和冠脉血流量显著下降。可见谷草转氨酶大量释放，且在较长时间缺血后更为明显。向灌注液中添加肾上腺素会增加酶的释放。以每分钟400次的频率起搏、高前负荷、高后负荷、酸中毒或碱中毒均未改变酶的释放。缺血期末糖原、三磷酸腺苷和磷酸肌酸水平降低，但在逆行灌注期间又开始上升。本研究表明，心肌组织可能在未受到不可逆损伤的情况下释放酶。
