通过非变性等电聚焦分离后对铜锌超氧化物歧化酶活性进行简单定量。
Simple quantification of Cu,Zn-superoxide dismutase activity after separation by non-denaturing isoelectric focusing.
作者信息
Shimazaki Youji, Ohnishi Hiromi, Matsuura Satoshi, Manabe Takashi
机构信息
Department of Material Science, Faculty of Science and Venture Business Laboratory, Ehime University, Matsuyama City, 790-8577 Japan.
出版信息
Biochim Biophys Acta. 2002 Jul 3;1571(3):245-8. doi: 10.1016/s0304-4165(02)00259-3.
The Cu,Zn-superoxide dismutase (SOD) activity in bovine retina cytosol was separated from retinal pigment using short-length non-denaturing isoelectric focusing (IEF) (15-mm long x 1.3-mm i.d. column) and detected using non-denaturing two-dimensional electrophoresis (2-DE). After the SOD and pigment in the retina cytosol are separated, SOD activity can be quantified by water-soluble tetrazolium salt. We also found that SOD separated by this IEF retained its native function.
利用短长度非变性等电聚焦（IEF）（15毫米长×1.3毫米内径柱）从牛视网膜细胞溶胶中分离出铜锌超氧化物歧化酶（SOD）活性，并与视网膜色素分离，然后使用非变性二维电泳（2-DE）进行检测。视网膜细胞溶胶中的SOD和色素分离后，可用水溶性四唑盐对SOD活性进行定量。我们还发现，通过这种IEF分离的SOD保留了其天然功能。
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