甘氨酸转运体亚型在PC12细胞中表现出不同的亚细胞定位。

Glycine transporter isoforms show differential subcellular localization in PC12 cells.

作者信息

Geerlings Arjan, Núñez Enrique, Rodenstein Lara, López-Corcuera Beatriz, Aragón Carmen

机构信息

Centro de Biología Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autónoma de Madrid, Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain.

出版信息

J Neurochem. 2002 Jul;82(1):58-65. doi: 10.1046/j.1471-4159.2002.00930.x.

DOI:10.1046/j.1471-4159.2002.00930.x

PMID:12091465

Abstract

The subcellular localization of glycine transporters one (GLYT1) and two (GLYT2) stably expressed in PC12 cells has been studied. To facilitate visualization, enhanced green fluorescent protein (GFP) was fused to the amino terminus of both glycine transporters. Functional analysis of the GFP-GLYT1 and GFP-GLYT2 stable cell lines demonstrated that they exhibited high affinity for glycine and the characteristic properties of both glycine transporter subtypes. The GFP-coupled transporters were differently distributed throughout the cell. GFP-GLYT1 was mainly localized on the plasma membrane, whereas most of GFP-GLYT2 was present on large dense-core vesicles and endosomes. Both transporters were absent from the synaptic vesicle population in PC12 cells.

摘要

已对稳定表达于PC12细胞中的甘氨酸转运体1（GLYT1）和甘氨酸转运体2（GLYT2）的亚细胞定位进行了研究。为便于观察，将增强型绿色荧光蛋白（GFP）融合至两种甘氨酸转运体的氨基末端。对GFP-GLYT1和GFP-GLYT2稳定细胞系的功能分析表明，它们对甘氨酸表现出高亲和力以及两种甘氨酸转运体亚型的特征性质。与GFP偶联的转运体在整个细胞中的分布不同。GFP-GLYT1主要定位于质膜，而大多数GFP-GLYT2存在于大型致密核心囊泡和内体上。PC12细胞的突触囊泡群体中均不存在这两种转运体。