Sithigorngul Paisarn, Rukpratanporn Sombat, Longyant Siwaporn, Chaivisuthangkura Parin, Sithigorngul Weerawan, Menasveta Piamsak
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok, Thailand.
Dis Aquat Organ. 2002 Apr 24;49(1):71-6. doi: 10.3354/dao049071.
Monoclonal antibodies specific to 22, 67 and 135 kDa proteins of yellow-head virus (YHV) were produced from a mouse immunized with partially purified YHV isolated from the haemolymph of experimentally YHV-infected Penaeus monodon. Four groups of monoclonal antibodies were identified. One group of antibodies bound only to native protein of YHV while the others were specific to 135, 67 and 22 kDa proteins in both native and denatured forms. All antibodies could be used to detect YHV infection by means of dot blot and immunohistochemistry. However, antibodies specific to the 22 kDa protein gave the best immunohistochemistry results in terms of intensity and sharpness of staining.
用从实验性感染黄头病毒（YHV）的斑节对虾血淋巴中分离出的部分纯化YHV免疫小鼠，制备了针对YHV 22 kDa、67 kDa和135 kDa蛋白的单克隆抗体。鉴定出了四组单克隆抗体。一组抗体仅与YHV的天然蛋白结合，而其他组抗体对天然和变性形式的135 kDa、67 kDa和22 kDa蛋白均具有特异性。所有抗体均可通过斑点印迹和免疫组织化学用于检测YHV感染。然而，就染色强度和清晰度而言，针对22 kDa蛋白的抗体在免疫组织化学中给出了最佳结果。
