Vrba M, Cíhalová V, Jurásková V
Research Institute of Child Health, Brno, 662 62 Czech Republic.
Neoplasma. 2002;49(3):184-8.
A permanent cell line [VUP] derived 31 years ago from human malignant melanoma of the choroid has been characterized by genetically firmly anchored heteronuclearity. The most significant chromosomal changes of this cell line are: high instability of the chromosome No. 13 with the rise of new chromosomes formed by translocations, homologous stability of chromosomes 6, 15, and X. Structural changes were not revealed in chromosomes 15 and 22. The variability of chromosomes was studied both by classical conventional methods as well as with GTG banding and DNA hybridization in situ (FISH). Structural diversity was demonstrated in a number of morphologically congruent chromosomes. For example, X chromosome classified morphologically as chromosome No. 10 was determined by means of FISH technique, as a centric fragment Xq with translocated acentric fragment of other chromosomes. Furthermore, mar-t, previously considered to be q arm of chromosome No. 4, is formed by a centric fragment of chromosome No. 13 and an acentric fragment of chromosome No. 1.
一个31年前从人类脉络膜恶性黑色素瘤中衍生出的永久细胞系[VUP]，其特征是具有遗传上稳定的异核性。该细胞系最显著的染色体变化为：13号染色体高度不稳定，出现由易位形成的新染色体；6号、15号染色体和X染色体具有同源稳定性。15号和22号染色体未发现结构变化。通过经典常规方法以及GTG显带和原位DNA杂交（FISH）研究了染色体的变异性。在许多形态上一致的染色体中显示出结构多样性。例如，通过FISH技术确定，形态上归类为10号染色体的X染色体是一个Xq着丝粒片段与其他染色体的无着丝粒易位片段。此外，之前被认为是4号染色体q臂的mar-t是由13号染色体的着丝粒片段和1号染色体的无着丝粒片段形成的。
