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由水稻腺苷酸激酶基因启动子调控的草铵膦耐受烟草植株。

Glufosinate-tolerant tobacco plants directed by the promoter of adenylate kinase gene of rice.

作者信息

Fukuzawa Hiromitsu, Arai Satoshi, Kawai-Yamada Maki, Das Avijit, Tagawa Michito, Uchimiya Hirofumi

机构信息

Department of Agricultural Chemistry, Shiraoka Research Station of Biological Science, Nissan Chemical Industries Ltd, Minamisaitama, Saitama, Japan.

出版信息

Ann Bot. 2002 Mar;89(3):351-4. doi: 10.1093/aob/mcf041.

DOI:10.1093/aob/mcf041
PMID:12099271
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4233820/
Abstract

A DNA clone containing the 5' part of the adenylate kinase (AK) gene was isolated from a rice genomic library, and its nucleotide sequence was determined. This clone consists of 5' upstream, five exons and four introns of the AK gene. All of the determined donor and receptor sites contained 'GT' and 'AG' consensus splice sequences. Transgenic tobacco plants harbouring a chimeric gene consisting of the 5' upstream sequence of the AK gene fused with the gene encoding phosphinothricin acetyl transferase were generated. They showed tolerance to glufosinate to a level four times higher than its commercial dose.

摘要

从水稻基因组文库中分离出一个包含腺苷酸激酶（AK）基因5'部分的DNA克隆，并测定了其核苷酸序列。该克隆由AK基因的5'上游、五个外显子和四个内含子组成。所有确定的供体和受体位点都含有“GT”和“AG”共有剪接序列。构建了一个嵌合基因，该基因由AK基因的5'上游序列与编码草丁膦乙酰转移酶的基因融合而成，并培育出了携带该嵌合基因的转基因烟草植株。这些植株对草铵膦的耐受性比商业剂量高四倍。

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