激活K-252b敏感蛋白激酶是大鼠海马体CA1区长期增强突触后阶段所必需的。

Activation of a K-252b-Sensitive Protein Kinase is Necessary for a Post-Synaptic Phase of Long-Term Potentiation in Area CA1 of Rat Hippocampus.

作者信息

Reymann K G, Davies S N, Matthies H, Kase H, Collingridge G L

机构信息

Department of Pharmacology, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK.

出版信息

Eur J Neurosci. 1990;2(6):481-6. doi: 10.1111/j.1460-9568.1990.tb00439.x.

DOI:10.1111/j.1460-9568.1990.tb00439.x

PMID:12106018

Abstract

K-252b, a potent inhibitor of protein kinases blocked a late phase of long-term potentiation (LTP) in area CA1 of rat hippocampal slices, resulting in decremental LTP. It also prevented the slowly developing increase in sensitivity of CA1 neurons to iontophoretically administered alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) which was seen in control slices that exhibit nondecremental LTP. However, K-252b applied 60 - 180 min after the induction of LTP had no effect on the potentiated synaptic and AMPA-induced responses. A K-252b-sensitive protein kinase may therefore be involved in a slowly developing postsynaptic component of LTP.

摘要

K-252b是一种强效蛋白激酶抑制剂，它能阻断大鼠海马切片CA1区的长时程增强（LTP）晚期阶段，导致LTP递减。它还能阻止CA1神经元对离子导入的α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）的敏感性缓慢增加，而在表现出非递减LTP的对照切片中可观察到这种增加。然而，在LTP诱导后60 - 180分钟应用K-252b对增强的突触反应和AMPA诱导的反应没有影响。因此，一种对K-252b敏感的蛋白激酶可能参与了LTP的缓慢发展的突触后成分。