Grabczyk Ed, Zuber Mauricio X., Federoff Howard J., Ng Shi-Chung, Pack Alison, Fishman Mark C.
Developmental Biology Laboratory, Massachusetts General Hospital, Department of Medicine, Harvard Medical School, and Howard Hughes Medical Institute, Boston, MA, USA.
Eur J Neurosci. 1990;2(10):822-827. doi: 10.1111/j.1460-9568.1990.tb00393.x.
GAP-43 is a gene expressed only in the nervous system. The protein product is believed to be important to neuronal growth and plasticity. Most, and likely all, neurons express high levels of GAP-43 during periods of neurite elongation. To initiate studies of GAP-43 gene regulation we have cloned the rat gene encoding GAP-43. The GAP-43 gene includes three exons. The first exon encodes only the amino terminal 10 amino acids, which corresponds to the membrane targeting domain of GAP-43. The second exon encodes a putative calmodulin binding domain and a protein kinase C phosphorylation site. The 5'-flanking sequence is unusual in that it lacks CAAT or TATA elements, and directs RNA transcription initiation from several sites. Some of the transcription start sites are used to a different degree in the central and peripheral nervous systems.
GAP - 43是一种仅在神经系统中表达的基因。其蛋白质产物被认为对神经元生长和可塑性很重要。大多数，甚至可能所有神经元在神经突伸长期间都高水平表达GAP - 43。为了启动对GAP - 43基因调控的研究，我们克隆了编码GAP - 43的大鼠基因。GAP - 43基因包含三个外显子。第一个外显子仅编码氨基末端的10个氨基酸，这对应于GAP - 43的膜靶向结构域。第二个外显子编码一个假定的钙调蛋白结合结构域和一个蛋白激酶C磷酸化位点。其5'侧翼序列不同寻常，因为它缺乏CAAT或TATA元件，并从多个位点指导RNA转录起始。一些转录起始位点在中枢和外周神经系统中的使用程度不同。
