疏水性和亲水性胆盐混合物对模拟胆汁中胆固醇结晶的影响。

Effects of hydrophobic and hydrophilic bile salt mixtures on cholesterol crystallization in model biles.

作者信息

Venneman Niels G, Huisman Sebastiaan J, Moschetta Antonio, vanBerge-Henegouwen Gerard P, van Erpecum Karel J

机构信息

Gastrointestinal Research Unit, Department of Gastroenterology, University Medical Center Utrecht, Utrecht, The Netherlands.

出版信息

Biochim Biophys Acta. 2002 Jul 11;1583(2):221-8. doi: 10.1016/s1388-1981(02)00216-0.

DOI:10.1016/s1388-1981(02)00216-0

PMID:12117566

Abstract

UNLABELLED

The hydrophilic bile salt ursodeoxycholate is frequently used to dissolve cholesterol gallstones. We have now quantitated crystallization as a function of bile salt hydrophobicity, phospholipid content, cholesterol saturation and total lipid concentration (TLCo).

METHODS

Crystallization in supersaturated model biles with low phospholipid contents (left two-phase-micelles and crystal-containing-zone) was assessed during 21 days by microscopy and chemical measurement of crystal mass. For model biles with higher phospholipid contents (central three-phase-micelles, vesicles and crystal-containing-zone), lipid distribution into various phases was determined by combined ultracentrifugation-filtration-dialysis methodology (Biochim. Biophys. Acta 1532 (2001) 15-27).

RESULTS

In the left two-phase zone, crystal numbers and masses were highest in case of more hydrophilic bile salt composition (TUDC 100%>TC/TUDC 70%/30%>TC 100%>TC/TDC 70%/30%>TDC 100%) and decreased with increasing phospholipid contents, lower TLCo and lower cholesterol saturation index (CSI). In contrast, in the presence of vesicles (three-phase zone), crystallization decreased at increasing bile salt hydrophilicity, with concomitant increased vesicular cholesterol solubilization.

CONCLUSIONS

Presence of vesicular phases is a prerequisite for inhibition of cholesterol crystallization by tauroursodeoxycholate.

摘要

未标记

亲水性胆盐熊去氧胆酸常用于溶解胆固醇胆结石。我们现在已将结晶量化为胆盐疏水性、磷脂含量、胆固醇饱和度和总脂质浓度（TLCo）的函数。

方法

通过显微镜检查和晶体质量的化学测量，在21天内评估低磷脂含量（左两相微团和含晶区）的过饱和模型胆汁中的结晶情况。对于磷脂含量较高（中间三相微团、囊泡和含晶区）的模型胆汁，通过超速离心-过滤-透析联合方法（《生物化学与生物物理学报》1532 (2001) 15 - 27）确定脂质在各相中的分布。

结果

在左两相区，晶体数量和质量在亲水性更强的胆盐组成情况下最高（牛磺熊去氧胆酸100%>牛磺胆酸/牛磺熊去氧胆酸70%/30%>牛磺胆酸100%>牛磺胆酸/去氧胆酸70%/30%>去氧胆酸100%），并随着磷脂含量增加、TLCo降低和胆固醇饱和指数（CSI）降低而减少。相反，在存在囊泡（三相区）的情况下，随着胆盐亲水性增加，结晶减少，同时囊泡中胆固醇的溶解增加。