Hepatic ischemia-reperfusion promotes liver metastasis of colon cancer.

作者信息

Doi Koji, Horiuchi Tetsuya, Uchinami Masaru, Tabo Takanori, Kimura Narisato, Yokomachi Jun, Yoshida Makoto, Tanaka Kuniyoshi

机构信息

Second Department of Surgery, Fukui Medical University, Fukui, Japan.

出版信息

J Surg Res. 2002 Jun 15;105(2):243-7. doi: 10.1006/jsre.2002.6356.

DOI:10.1006/jsre.2002.6356

PMID:12121713

Abstract

BACKGROUND

The effects of hepatic ischemia/reperfusion (I/R) on liver metastasis have not been fully examined. We examined hepatic I/R and liver metastasis of colorectal cancer in a rat model; we also quantitated expression of E-selectin (ELAM-1) mRNA after I/R.

MATERIALS AND METHODS

Rats underwent 30 or 60 min of 70% partial hepatic ischemia. After 60 min of reperfusion, rat colon adenocarcinoma cells (RCN-H4) were inoculated intrasplenically. The number of tumor nodules on the liver surface was determined 3 weeks later. Expression of E-selectin mRNA was determined at 1, 3, and 6 h after ischemia by quantitative RT-PCR.

RESULTS

Hepatic I/R promoted liver metastasis of RCN-H4 and induced the expression of E-selectin mRNA in both clamped and unclamped liver lobes. The number of tumor nodules and the expression of E-selectin mRNA after 60 min of ischemia was greater than that after 30 min.