泰国人群中基质细胞衍生因子（SDF）1 - 3'A多态性及序列

Stromal cell-derived factor (SDF) 1-3'A polymorphism and sequences in Thais.

作者信息

Tiensiwakul Pornthep

机构信息

Department of Clinical Microscopy, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.

出版信息

Asian Pac J Allergy Immunol. 2002 Mar;20(1):61-5.

PMID:12125920

Abstract

Stromal cell derived factor (SDF) 1-3'A polymorphism in Thai subjects was determined by restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR) products using a restriction enzyme Msp 1 cleavage. The allelic frequency of SDF1-3'A in this population was 0.289. SDF1-3'A genotyping showed 9.52% SDF1-3'A/3'A, 38.89% SDF1-wt/3'A, and 51.59% SDF1-wt/wt. Two clones of Thai-SDF genes, BD41 and BD42, were isolated and sequenced. Wild type Thai-SDF1 (BD42), a 278 bp sequence was identically aligned to human pre B cell stimulating factor homoloque (SDF1-b) (GenBank accession number L-36033) from nucleotides 788 to 1,065. Homologous Thai SDF1-3'A (BD41), a 277 bp sequence differed by single nucleotide with adenine substitution to guanine at position 880 of the SDF1-b, is the first evidence of SDF1-3'A polymorphism in Thais.

摘要

通过使用限制性内切酶Msp 1切割聚合酶链反应（PCR）产物的限制性片段长度多态性（RFLP），确定泰国受试者中基质细胞衍生因子（SDF）1-3'A多态性。该人群中SDF1-3'A的等位基因频率为0.289。SDF1-3'A基因分型显示9.52%为SDF1-3'A/3'A、38.89%为SDF1-wt/3'A，以及51.59%为SDF1-wt/wt。分离并测序了两个泰国SDF基因克隆，BD41和BD42。野生型泰国SDF1（BD42），一个278 bp的序列，从核苷酸788到1065与人类前B细胞刺激因子同源物（SDF1-b）（GenBank登录号L-36033）完全比对。同源的泰国SDF1-3'A（BD41），一个277 bp的序列，在SDF1-b的第880位核苷酸处因单个核苷酸差异，腺嘌呤被鸟嘌呤取代，这是泰国人SDF1-3'A多态性的首个证据。