棕色固氮菌中cbb3型末端氧化酶的运作
Operation of the cbb3-type terminal oxidase in Azotobacter vinelandii.
作者信息
Bertsova Yu V, Bogachev A V
机构信息
Department of Bioenergetics, Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia.
出版信息
Biochemistry (Mosc). 2002 Jun;67(6):622-6. doi: 10.1023/a:1016185919112.
A part of the gene encoding cbb3-type cytochrome oxidase CcoN subunit was cloned from Azotobacter vinelandii and a mutant strain of this bacterium with disrupted ccoN gene was constructed. In contrast to the wild type strain, this one is unable to oxidize cytochromes c4 and c5. Thus, the A. vinelandii respiratory chain is shown to contain cbb3-type cytochrome c oxidase. It is also shown that the activity of this enzyme is not necessary for diazotrophic growth of A. vinelandii at high oxygen concentrations.
从棕色固氮菌中克隆出编码cbb3型细胞色素氧化酶CcoN亚基的部分基因，并构建了该细菌ccoN基因被破坏的突变菌株。与野生型菌株相比，该突变菌株无法氧化细胞色素c4和c5。因此，证明棕色固氮菌的呼吸链中含有cbb3型细胞色素c氧化酶。还表明，在高氧浓度下，该酶的活性对于棕色固氮菌的固氮生长并非必需。
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