表达膜型巨噬细胞集落刺激因子的T9胶质瘤细胞可产生针对T9颅内胶质瘤的CD4 + T细胞相关保护性免疫以及针对不同同基因胶质瘤的全身免疫。

T9 glioma cells expressing membrane-macrophage colony stimulating factor produce CD4+ T cell-associated protective immunity against T9 intracranial gliomas and systemic immunity against different syngeneic gliomas.

作者信息

Sanchez Ramon, Williams Christopher, Daza Jose L, Dan Qinghong, Xu Qingcheng, Chen Yijun, Delgado Christina, Arpajirakul Neary, Jeffes Edward W B, Kim Ronald C, Douglass Thomas, Al Atar Usama, Terry Wepsic H, Jadus Martin R

机构信息

Diagnostic and Molecular Health Care Group, Box 113 Veterans Affairs Medical Center, 5901 E. 7th Street, Long Beach, CA 90822, USA.

出版信息

Cell Immunol. 2002 Jan;215(1):1-11. doi: 10.1016/s0008-8749(02)00011-4.

DOI:10.1016/s0008-8749(02)00011-4

PMID:12142031

Abstract

Cloned T9 glioma cells (T9-C2) expressing the membrane form of macrophage colony stimulating factor (mM-CSF) inoculated subcutaneously into rats do not grow and glioma-specific immunity is stimulated. Immunotherapy experiments showed that intracranial T9 tumors present for one to four days could be successfully eradicated by peripheral vaccination with T9-C2 cells. CD4+ and CD8+ T splenocytes from immunized rats, when restimulated in vitro with T9 cells, produced interleukin-2 and -4. Protective immunity against intracranial T9 gliomas could only be adoptively transferred into naive rats by the CD4+ splenocytes obtained from T9-C2 immunized rats. Rats immunized by the T9-C2 tumor cells also resisted two different syngeneic gliomas (RT2 and F98) but allowed a syngeneic NUTU-19 ovarian cancer to grow. Such cross-protective immunity against unrelated gliomas suggests that mM-CSF transfected tumor cells have immunotherapeutic potential for use as an allogeneic tumor vaccine.

摘要

将表达巨噬细胞集落刺激因子膜形式（mM-CSF）的克隆T9胶质瘤细胞（T9-C2）皮下接种到大鼠体内后，肿瘤不会生长，且会刺激胶质瘤特异性免疫。免疫治疗实验表明，外周接种T9-C2细胞可成功根除已存在1至4天的颅内T9肿瘤。用T9细胞体外再次刺激免疫大鼠的CD4+和CD8+脾细胞时，这些细胞会产生白细胞介素-2和-4。对颅内T9胶质瘤的保护性免疫只能通过从T9-C2免疫大鼠获得的CD4+脾细胞过继转移到未免疫大鼠体内。用T9-C2肿瘤细胞免疫的大鼠也能抵抗两种不同的同基因胶质瘤（RT2和F98），但同基因的NUTU-19卵巢癌却能生长。这种对不相关胶质瘤的交叉保护性免疫表明，转染了mM-CSF的肿瘤细胞具有作为同种异体肿瘤疫苗的免疫治疗潜力。