从海星精子头部分离肌球蛋白。

Isolation of myosin from starfish sperm heads.

作者信息

Mabuchi I

出版信息

J Biochem. 1976 Aug;80(2):413-5. doi: 10.1093/oxfordjournals.jbchem.a131293.

DOI:10.1093/oxfordjournals.jbchem.a131293

Abstract

Myosin was extracted and partially purified from the head portion of spermatozoa of the starfish, Asterias amurensis. The sperm myosin showed a specific Ca2+-activated ATPase [EC 3.6.1.3] activity of 0.2 mumoles Pi/min/mg at high ionic strength and pH 6.5. It resembled egg myosin in forming thick filaments, becoming attached to actin filaments. subunit composition, and serological properties.

摘要

从多棘海盘车精子头部提取并部分纯化了肌球蛋白。在高离子强度和pH 6.5条件下，精子肌球蛋白表现出特定的Ca2+激活的ATP酶[EC 3.6.1.3]活性，为0.2微摩尔无机磷/分钟/毫克。它在形成粗丝、附着于肌动蛋白丝、亚基组成和血清学特性方面与卵肌球蛋白相似。