丝氨酸蛋白酶神经蛋白酶（KLK8）在正常和病理皮肤样本中的表皮表达。
Epidermal expression of serine protease, neuropsin (KLK8) in normal and pathological skin samples.
作者信息
Kuwae K, Matsumoto-Miyai K, Yoshida S, Sadayama T, Yoshikawa K, Hosokawa K, Shiosaka S
机构信息
Department of Plastic Surgery, Osaka University Medical School, Osaka, Japan.
出版信息
Mol Pathol. 2002 Aug;55(4):235-41. doi: 10.1136/mp.55.4.235.
AIMS
The expression of human neuropsin (KLK8) mRNA in normal and pathological skin samples was analysed and the results compared with those for tissue plasminogen activator (tPA) mRNA.
METHODS
Northern blot and in situ hybridisation analyses of KLK8 mRNA in normal and lesional skin of patients with cutaneous diseases were performed.
RESULTS
A weak signal for KLK8 mRNA and no signal for tPA mRNA was seen in normal skin on northern blot analysis. Weak signals for KLK8 were localised to the superficial cells beneath the cornified layer in normal skin on in situ hybridisation. Psoriasis vulgaris, seborrheic keratosis, lichen planus, and squamous cell carcinoma skin samples, which show severe hyperkeratosis, displayed a high density of KLK8 mRNA on northern and in situ hybridisation analyses. The signals were localised in granular and spinous layers of lesional skin in all hyperkeratic samples, including the area surrounding the horn pearls of squamous cell carcinoma. To examine the relation between mRNA expression and terminal differentiation, the expression of KLK8 mRNA was analysed in cell cultures. When keratinisation proceeded in high calcium medium, a correlative increase in the expression of KLK8 mRNA was observed.
CONCLUSION
The results are consistent with a role for this protease in the terminal differentiation of keratinocytes.
目的
分析人神经蛋白酶（KLK8）mRNA在正常和病理皮肤样本中的表达，并将结果与组织纤溶酶原激活物（tPA）mRNA的结果进行比较。
方法
对皮肤疾病患者正常皮肤和皮损处皮肤的KLK8 mRNA进行Northern印迹和原位杂交分析。
结果
Northern印迹分析显示，正常皮肤中KLK8 mRNA信号较弱，tPA mRNA无信号。原位杂交显示，正常皮肤中KLK8的弱信号定位于角质层下方的表层细胞。寻常型银屑病、脂溢性角化病、扁平苔藓和鳞状细胞癌皮肤样本均显示严重角化过度，Northern印迹和原位杂交分析显示其KLK8 mRNA密度较高。在所有角化过度样本中，包括鳞状细胞癌角珠周围区域，信号定位于皮损处皮肤的颗粒层和棘层。为了研究mRNA表达与终末分化之间的关系，在细胞培养中分析了KLK8 mRNA的表达。当在高钙培养基中发生角质化时，观察到KLK8 mRNA表达呈相关增加。
结论
这些结果与这种蛋白酶在角质形成细胞终末分化中的作用一致。
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