Mittenberg A G, Kulichkova V A, Medvedeva N D, Volkova I V, Ermolaeva Iu B, Konstantinova I M
Institute of Cytology RAS, St. Petersburg.
Tsitologiia. 2002;44(4):357-63.
The ability of 26S proteasomes from the human proerythroleukaemic cell line K562 to degrade high-molecular-weight cytoplasmic RNAs, particularly specific messenger RNA, has been detected. The addition of hemin to K562 cells in the culture media leads to redistribution of proteasomes and their migration mainly to the cytoplasm. The human wild type p53 gene mRNA was shown to be specifically nucleolized by proteasomes. These particles displayed endoribonuclease activity towards mRNA for Renilla sp. luciferase. Proteasomes also specifically degraded Alu-containing mRNAs. A supposition is made about the involvement of proteasomes in stability control of specific RNA groups.
已检测到人早幼粒白血病细胞系K562的26S蛋白酶体降解高分子量细胞质RNA，特别是特定信使RNA的能力。在培养基中向K562细胞添加血红素会导致蛋白酶体重新分布，并主要迁移至细胞质。已表明人野生型p53基因mRNA被蛋白酶体特异性核仁化。这些颗粒对海肾荧光素酶的mRNA表现出核糖核酸内切酶活性。蛋白酶体还能特异性降解含Alu的mRNA。推测蛋白酶体参与特定RNA组的稳定性控制。
