Belitsky Jason M, Leslie Stephanie J, Arora Paramjit S, Beerman Terry A, Dervan Peter B
Division of Chemistry and Chemical Engineering and Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA.
Bioorg Med Chem. 2002 Oct;10(10):3313-8. doi: 10.1016/s0968-0896(02)00204-3.
The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide-dye conjugates in a variety of living cells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, polyamide-dye conjugates localize mainly in the cytoplasm and not in the nucleus. Reagents such as methanol typically used to fix cells for microscopy significantly alter the cellular localization of these DNA-binding ligands.
通过共聚焦激光扫描显微镜检查了DNA结合的N-甲基吡咯/N-甲基咪唑聚酰胺-染料共轭物在多种活细胞中的细胞摄取和定位特性。除了某些T细胞系外，聚酰胺-染料共轭物主要定位于细胞质而非细胞核。通常用于固定细胞以进行显微镜检查的试剂，如甲醇，会显著改变这些DNA结合配体的细胞定位。
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