Bekasova O D, Shubin L M, Evstigneev V B
Biol Bull Acad Sci USSR. 1979 Mar-Apr;6(2):164-72.
Phycobilisomes (PBS) were isolated from Aphanizomenon flos-aquae and Anabaena variabilis. The absorption spectra and second derivative of the absorption spectra of isolated PBS indicate the presence of phycoerythrocyanin, and allophycocyanin. The fluorescence spectra of PBS were measured at room temperature and -196 degrees C. Undamaged PBS have the principal fluorescence maximum in the region of 660 nm at room temperature and in the region of 685-690 nm at -196 degrees. When the PBS were heated from ) to 60 degrees the fluorescence at 685-690 nm disappeared and it increased in the region of 650-660 nm. This is apparently due to disruption of the structure of PBS, which results in a disturbance in the migration of energy along the chain phycoerythrocyanin leads to phycocyanin leads to allophycocyanin.
从水华鱼腥藻和多变鱼腥藻中分离出藻胆体（PBS）。分离出的藻胆体的吸收光谱及其吸收光谱的二阶导数表明存在藻红蛋白和别藻蓝蛋白。在室温及-196℃下测量了藻胆体的荧光光谱。未受损的藻胆体在室温下主要荧光峰在660nm区域，在-196℃时在685 - 690nm区域。当藻胆体从0℃加热到60℃时，685 - 690nm处的荧光消失，而在650 - 660nm区域荧光增强。这显然是由于藻胆体结构的破坏，导致能量沿藻红蛋白→藻蓝蛋白→别藻蓝蛋白链迁移受到干扰。
