BALB/c小鼠对偏硅酸钠的超敏反应

Sodium metasilicate hypersensitivity in BALB/c mice.

作者信息

Karrow N A, Guo T L, Leffel E K, Zhang L X, McCay J A, Germolec D R, White K L

机构信息

Department of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA 23298, USA.

出版信息

Am J Contact Dermat. 2002 Sep;13(3):133-9.

PMID:12165932

Abstract

BACKGROUND

Sodium metasilicate (SMS) is a key ingredient for a number of industrial and consumer products. Although little is known about potential for this chemical to cause allergic reactions, a similar silicate compound, sodium silicate, was reported to elicit IgE-mediated contact urticaria.

OBJECTIVE

The aim of this study was to evaluate the potential for sodium metasilicate to elicit an allergic response in female BALB/c mice after dermal exposure.

METHODS

The primary irritancy assay (IA), local lymph node assay (LLNA), and a mouse ear swelling test (MEST) were used to evaluate the hypersensitivity response elicited by SMS exposure. An evaluation of lymph node subpopulations, cytokine mRNA expression, and serum IgE levels was also conducted.

RESULTS

SMS caused significant dermal irritation at concentrations >or=6% and an allergic response after mice were sensitized with 4% SMS then challenged with 6% SMS in the MEST. Lymph node cell proliferation was not observed in the LLNA after treatment with SMS (2% to 6% SMS). Increases in lymph node cellularity, the percentage of B cells, and the expression of certain cytokine mRNAs were observed in mice treated with SMS. Changes in the concentration of serum IgE after SMS treatment, however, were not observed.

CONCLUSIONS

SMS appears to elicit a chemical hypersensitivity response in mice, as indicated by the MEST, but not by the LLNA. Increases in auricular lymph node cellularity, the percentage of B cells, and certain cytokine mRNAs support classifying SMS as a weak chemical allergen.

摘要

背景

偏硅酸钠（SMS）是许多工业和消费品的关键成分。尽管对于这种化学物质引起过敏反应的可能性知之甚少，但据报道，一种类似的硅酸盐化合物硅酸钠可引发IgE介导的接触性荨麻疹。

目的

本研究旨在评估偏硅酸钠经皮肤暴露后在雌性BALB/c小鼠中引发过敏反应的可能性。

方法

采用初级刺激性试验（IA）、局部淋巴结试验（LLNA）和小鼠耳肿胀试验（MEST）来评估暴露于SMS引发的超敏反应。还对淋巴结亚群、细胞因子mRNA表达和血清IgE水平进行了评估。

结果

在MEST中，当浓度≥6%时，SMS会引起明显的皮肤刺激，在用4% SMS致敏小鼠后再用6% SMS进行激发时会引发过敏反应。用SMS（2%至6% SMS）处理后，LLNA中未观察到淋巴结细胞增殖。在用SMS处理的小鼠中观察到淋巴结细胞增多、B细胞百分比增加以及某些细胞因子mRNA表达增加。然而，未观察到SMS处理后血清IgE浓度的变化。