从印加花生（Plukenetia volubilis L.）中分离、纯化一种新型水溶性蛋白质及其生化特性分析

Isolation, purification, and biochemical characterization of a novel water soluble protein from Inca peanut (Plukenetia volubilis L.).

作者信息

Sathe Shridhar K, Hamaker Bruce R, Sze-Tao Kar Wai Clara, Venkatachalam Mahesh

机构信息

Department of Nutrition, Food and Exercise Sciences, College of Human Sciences, Florida State University, Tallahassee, Florida 32306-1493, USA.

出版信息

J Agric Food Chem. 2002 Aug 14;50(17):4906-8. doi: 10.1021/jf020126a.

DOI:10.1021/jf020126a

PMID:12166980

Abstract

A water soluble storage albumin from Inca peanut (IPA) accounted for approximately 25% (w/w) of defatted seed flour weight, representing 31% of the total seed protein. IPA is a 3S storage protein composed of two glycosylated polypeptides, with estimated molecular weights (MW) of 32800 and 34800 Da, respectively. IPA has an estimated sugar content of 4.8% +/- 0.92% (n = 6). IPA is a basic protein (pI of approximately 9.4) and contains all of the essential amino acids in adequate amounts when compared to the FAO/WHO recommended pattern for a human adult. The tryptophan content of IPA is unusually high (44 mg/g of protein), whereas the phenylalanine content is low (9 mg/g of protein). IPA is a highly digestible protein in vitro.

摘要

来自印加花生（IPA）的一种水溶性储存白蛋白约占脱脂种子粉重量的25%（w/w），占种子总蛋白的31%。IPA是一种3S储存蛋白，由两种糖基化多肽组成，估计分子量（MW）分别为32800和34800 Da。IPA的含糖量估计为4.8%±0.92%（n = 6）。IPA是一种碱性蛋白（pI约为9.4），与粮农组织/世界卫生组织推荐的成人模式相比，含有足够量的所有必需氨基酸。IPA的色氨酸含量异常高（44 mg/g蛋白质），而苯丙氨酸含量低（9 mg/g蛋白质）。IPA在体外是一种高度可消化的蛋白质。