杀蚊幼虫芽孢杆菌属中肠毒素基因的检测

Detection of enterotoxin genes in mosquito-larvicidal Bacillus species.

作者信息

Yuan Zhiming, Hansen Bjarne M, Andrup Lars, Eilenberg Jørgen

机构信息

Wuhan Institute of Virology, Chinese Academy of Sciences, China.

出版信息

Curr Microbiol. 2002 Sep;45(3):221-5. doi: 10.1007/s00284-001-0105-6.

DOI:10.1007/s00284-001-0105-6

PMID:12177746

Abstract

The presence of the B component of hemolysin BL (hblA), enterotoxin BceT (bceT), and enterotoxin S (entS) genes in mosquito-larvicidal Bacillus sp., including 25 B. sphaericus and 4 B. thuringiensis subsp. israelensis strains, has been analyzed by multiplex PCR in this study. The results showed that all four B. thuringiensis strains contain the hblA gene and the sequences of bceT and entS genes. However, none of the enterotoxin gene sequences were detected in the B. sphaericus strains. The enterotoxin production in all strains has also been analyzed by using two commercial immunoassay kits (TECRA and RPLA), and it has been proved that all the B. thuringiensis strains and one B. cereus strain can produce enterotoxins during growth. No enterotoxin activity could be detected in B. sphaericus strains.

摘要

本研究通过多重聚合酶链反应（multiplex PCR）分析了杀蚊幼虫芽孢杆菌中溶血素BL（hblA）的B组分、肠毒素BceT（bceT）和肠毒素S（entS）基因的存在情况，这些菌株包括25株球形芽孢杆菌和4株苏云金芽孢杆菌以色列亚种。结果表明，所有4株苏云金芽孢杆菌均含有hblA基因以及bceT和entS基因序列。然而，在球形芽孢杆菌菌株中未检测到任何肠毒素基因序列。还使用两种商业免疫分析试剂盒（TECRA和RPLA）分析了所有菌株的肠毒素产生情况，结果证明所有苏云金芽孢杆菌菌株和1株蜡样芽孢杆菌菌株在生长过程中均可产生肠毒素。在球形芽孢杆菌菌株中未检测到肠毒素活性。